The ability to study bacteria at the single cell level has advanced our insights into microbial physiology and genetics in ways not attainable by studying large populations using more traditional culturing methods. To improve methods to characterize bacteria at the cellular level, we developed a new microfluidic platform that enables cells to be exposed to metabolites in a gradient of concentrations. By designing low-cost, three-dimensional devices with adhesive tapes and tailoring them for bact...

Electric Engineering  Biological Materials  Mycology  Biological Chemistry  Veterinary Medicine  Molecular Biology  Toxicology  Computer Engineering  Microbiology  Biochemistry  Agricultural Engineering  Biodiversity  Developmental Biology  Bioengineering  Environmental Sciences  Environmental Sciences  Biological Engineering  Biotechnology  Chemical Engineering & Technology  Hydrology  Genomics  Genetics  Food Science & Technology  Analytical Chemistry 

Doi:10.1021/acssensors.9b01031

The soybean cyst nematode (SCN), Heterodera glycines, is the most damaging pathogen of soybeans in the United States. To assess the severity of nematode infestations in the field, SCN egg population densities are determined. Cysts (dead females) of the nematode must be extracted from soil samples and then ground to extract the eggs within. Sucrose centrifugation commonly is used to separate debris from suspensions of extracted nematode eggs. We present a method using OptiPrep as a density gradie...

Veterinary Medicine  Taxonomy  Automata  Agricultural Engineering  Environmental Sciences  Environmental Sciences  Animal Behavior  Agricultural Science  Biological Engineering  Genomics  Chemical Engineering & Technology  Genetics  Food Science & Technology  Electric Engineering  Industrial Engineering  Computer Engineering  Biochemistry  Biodiversity  Bioengineering  Geology  Hydrology  Parasitology  Plant Science  Soil Science  Synthetic Biology  Agronomy 

Doi:10.1371/journal.pone.0223386

A hallmark of bacterial populations cultured in vitro is their homogeneity of growth, where the majority of cells display identical growth rate, cell size and content. Recent insights, however, have revealed that even cells growing in exponential growth phase can be heterogeneous with respect to variables typically used to measure cell growth. Bacterial heterogeneity has important implications for how bacteria respond to environmental stresses, such as antibiotics. The phenomenon of antimicrobia...

Ecology  Developmental Biology  Agricultural Engineering  Environmental Sciences  Artificial Intelligence  Biological Engineering  Genomics  Chemical Engineering & Technology  Genetics  Food Science & Technology  Diagnostics  Biological Materials  Electric Engineering  Genetic Engineering  Industrial Engineering  Computer Engineering  Health Policy  Biochemistry  Biodiversity  Bioengineering  Bioinformatics  Biophysics  Biotechnology  Computational Biology  Cell Biology  Infectious Diseases  Immunology  Internal Medicine  Metabolic Sciences  Medical Genetics  Allergy & Clinical Immunology  Zoology  Biological Chemistry  Applied Physics  Taxonomy  Medicinal Chemistry  Agricultural Science  Analytical Chemistry  Mycology  Public Health  Mathematical Biology  Marine Biology  Molecular Biology  Microbiology  Global Health  Parasitology  Plant Science  Translational Medicine  Soil Science  Synthetic Biology  Materials Engineering 

Doi:10.1016/j.ohx.2021.e00249

Population-scale and rapid testing for SARS-CoV-2 continues to be a priority for several parts of the world. We revisit the in vitro technology platforms for COVID-19 testing and diagnostics—molecular tests and rapid antigen tests, serology or antibody tests, and tests for the management of COVID-19 patients. Within each category of tests, we review the commercialized testing platforms, their analyzing systems, specimen collection protocols, testing methodologies, supply chain logistics, and rel...

Allergy & Clinical Immunology  Electrochemistry  Information Technologies  Medical Education  Biological Chemistry  Virology  Medicinal Chemistry  Developmental Biology  Environmental Sciences  Biological Engineering  Genomics  Genetics  Food Science & Technology  Marketing  Oncology  Technology And Innovation  Theoretical Chemistry  Diagnostics  Supply Chain Management  Biological Materials  Nanometer Materials  Drugs & Devices  Public Health  Organic Chemistry  Epidemiology  Sales Management  Molecular Biology  Health Policy  Microbiology  Global Health  Bioengineering  Physical Chemistry  Bioinformatics  Biotechnology  Functional Materials  Computational Biology  Cell Biology  Parasitology  Translational Medicine  Infectious Diseases  Women’s Health  Immunology  Respiratory Medicine  Materials Engineering  Internal Medicine  Metabolic Sciences  Medical Genetics 

Doi:10.3390/s21196581

Diabetes mellitus (DM) is a serious health problem in Indonesia. Type 2 diabetes is a metabolic disease which primarily caused by obesity-linked insulin resistance. There is a link between insulin resistance and bacterial populations in the gut. Therefore, the aims of this study was to analyze composition of bacteria belong to the phylum Firmicutes and Bacteroidetes in women with type 2 diabetes and non-diabetic persons as control. The study included five female adults type 2 diabetes patients a...

Diabetes & Endocrinology  Genomics  Microbiology 

Doi:10.4308/hjb.26.3.110

The methonolic extract of the leaves of Pisonia alba (PA) were subjected to phytochemical analysis by standard qualitative analysis and the invitro antioxidant activity was evaluated by determination of total antioxidant capacity, 1.1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, Antioxidant activity, Nitric oxide potential. The analyses revealed that the methanol extract of Co was able to efficiently scavenge the free radicals in a dose dependant manner. The results were compare...

Zoology  Virology  Ecology  Ecosystem Science  Conservation Biology  Developmental Biology  Evolutionary Studies  Entomology  Environmental Sciences  Animal Behavior  Agricultural Science  Histology  Genomics  Genetics  Food Science & Technology  Anthropology  Aquaculture, Fisheries & Fish Science  Biochemistry  Biodiversity  Bioengineering  Biogeography  Bioinformatics  Biophysics  Biotechnology  Computational Biology  Cell Biology 

Doi:10.5281/zenodo.1312689

Background Typhoid fever is an infectious disease of global importance that is caused by Salmonella enterica subsp. enterica serovar Typhi (S. Typhi). This disease causes an estimated 200,000 deaths per year and remains a serious global health threat. S. Typhi is strictly a human pathogen, and some recovered individuals become long-term carriers who continue to shed the bacteria in their faeces, thus becoming main reservoirs of infection. Results A comparative genomics analysis combined with a ...

Epidemiology  Molecular Biology  Microbiology  Information retrieval  Evolutionary Studies  High Performance Computing  Biotechnology  Genomics  Computational Biology  Genetics  Infectious Diseases  Pathology  Medical Genetics