The ability to study bacteria at the single cell level has advanced our insights into microbial physiology and genetics in ways not attainable by studying large populations using more traditional culturing methods. To improve methods to characterize bacteria at the cellular level, we developed a new microfluidic platform that enables cells to be exposed to metabolites in a gradient of concentrations. By designing low-cost, three-dimensional devices with adhesive tapes and tailoring them for bact...

Electric Engineering  Biological Materials  Mycology  Biological Chemistry  Veterinary Medicine  Molecular Biology  Toxicology  Computer Engineering  Microbiology  Biochemistry  Agricultural Engineering  Biodiversity  Developmental Biology  Bioengineering  Environmental Sciences  Environmental Sciences  Biological Engineering  Biotechnology  Chemical Engineering & Technology  Hydrology  Genomics  Genetics  Food Science & Technology  Analytical Chemistry 

Doi:10.1021/acssensors.9b01031

The soybean cyst nematode (SCN), Heterodera glycines, is the most damaging pathogen of soybeans in the United States. To assess the severity of nematode infestations in the field, SCN egg population densities are determined. Cysts (dead females) of the nematode must be extracted from soil samples and then ground to extract the eggs within. Sucrose centrifugation commonly is used to separate debris from suspensions of extracted nematode eggs. We present a method using OptiPrep as a density gradie...

Veterinary Medicine  Taxonomy  Automata  Agricultural Engineering  Environmental Sciences  Environmental Sciences  Animal Behavior  Agricultural Science  Biological Engineering  Genomics  Chemical Engineering & Technology  Genetics  Food Science & Technology  Electric Engineering  Industrial Engineering  Computer Engineering  Biochemistry  Biodiversity  Bioengineering  Geology  Hydrology  Parasitology  Plant Science  Soil Science  Synthetic Biology  Agronomy 

Doi:10.1371/journal.pone.0223386

Soybeans are an important crop for global food security. Every year, soybean yields are reduced by numerous soybean diseases, particularly the soybean cyst nematode (SCN). It is difficult to visually identify the presence of SCN in the field, let alone its population densities or numbers, as there are no obvious aboveground disease symptoms. The only definitive way to assess SCN population densities is to directly extract the SCN cysts from soil and then extract the eggs from cysts and count the...

Electric Engineering  Computer Engineering  Ecosystem Science  Technology  Biodiversity  Agricultural Engineering  Bioengineering  Environmental Sciences  Artificial Intelligence  Agricultural Science  Biological Engineering  Food Science & Technology  Parasitology  Plant Science  Soil Science  Agronomy 

Doi:10.1038/s41598-021-82261-w

A hallmark of bacterial populations cultured in vitro is their homogeneity of growth, where the majority of cells display identical growth rate, cell size and content. Recent insights, however, have revealed that even cells growing in exponential growth phase can be heterogeneous with respect to variables typically used to measure cell growth. Bacterial heterogeneity has important implications for how bacteria respond to environmental stresses, such as antibiotics. The phenomenon of antimicrobia...

Ecology  Developmental Biology  Agricultural Engineering  Environmental Sciences  Artificial Intelligence  Biological Engineering  Genomics  Chemical Engineering & Technology  Genetics  Food Science & Technology  Diagnostics  Biological Materials  Electric Engineering  Genetic Engineering  Industrial Engineering  Computer Engineering  Health Policy  Biochemistry  Biodiversity  Bioengineering  Bioinformatics  Biophysics  Biotechnology  Computational Biology  Cell Biology  Infectious Diseases  Immunology  Internal Medicine  Metabolic Sciences  Medical Genetics  Allergy & Clinical Immunology  Zoology  Biological Chemistry  Applied Physics  Taxonomy  Medicinal Chemistry  Agricultural Science  Analytical Chemistry  Mycology  Public Health  Mathematical Biology  Marine Biology  Molecular Biology  Microbiology  Global Health  Parasitology  Plant Science  Translational Medicine  Soil Science  Synthetic Biology  Materials Engineering 

Doi:10.1016/j.ohx.2021.e00249

Precision swine production can benefit from autonomous, noninvasive, and affordable devices that conduct frequent checks on the well-being status of pigs. Here, we present a remote monitoring tool for the objective measurement of some behavioral indicators that may help in assessing the health and welfare status—namely, posture, gait, vocalization, and external temperature. The multiparameter electronic sensor board is characterized by laboratory measurements and by animal tests. Relevant behavi...

Software Engineering  Information Technologies  Zoology  Veterinary Medicine  Information Science  Economics  Automata  Technology  Agricultural Engineering  Communication Protocols  Environmental Sciences  Cloud Computing  Animal Behavior  Artificial Intelligence  Mobile and Portable Communications Systems  Agricultural Science  Big Data Search and Mining  Mobile and Ubiquitous Networks  Information Management  Applications of Communication Systems  Biological Engineering  Chemical Engineering & Technology  Mobile Computing Systems  Food Science & Technology  Logistics Engineering  Technology And Innovation  Machine Learning  Electric Engineering  Biological Materials  Public Health  Industrial Engineering  Statistics  Computer Engineering  Information retrieval  Biodiversity  Bioengineering  Image Processing  Distributed computing  Computer Vision  Consumer Behavior  Entrepreneurship 

Doi:10.3390/ani11092665

Banana is one of the leading fruit commodities of Indonesia and ranks the sixth position as one of the largest banana producers in the world. There are more than 200 types of banana in Indonesia. The utilization of bananas is in？uenced by the local culture, where in every 10 horticultural household, 5 of them plant bananas both as garden plants or feld plants. This horticultural crop is expectantly being one of the actions to improve economic prosperity especially in rural areas. In maintaining ...

Plant Science  Biodiversity 

Doi:10.4308/hjb.26.1.7

Ciliwung riverbank has been a？ected by settlement occupation. Both concrete and another retaining wall system have been built to avoid ？ood and erosion to the settlement that has existed adjacent to the river. Built environment as human disturbance can trigger the change of species richness of ground vegetation in the Ciliwung riverbank of Bogor City. The research objective is to study the di？erence of ground vegetation biodiversity in three di？erent types of riverbank based on its condition: na...

Plant Science  Ecosystem Science  Biodiversity  Environmental Sciences 

Doi:10.4308/hjb.26.1.35

Sea snail gonggong is an icon of Tanjungpinang-Riau Islands Province. It is a favorite seafood item in Riau Islands Province, and is high economic value but not known widely yet. Until now, sea snail gonggong has been highly exploited but the research on this snail is very limited. The aim of this study was to morphology and molecular characterization of Bintan gonggong snail (Strombus sp.) as a species validation. Bintan gonggong snail included thick-shelled gonggong and thin-shelled gonggong. ...

Taxonomy  Biodiversity 

Doi:10.4308/hjb.26.2.56

From revious research reported that tropical peatland is one of terrestrial carbon storage in Earth, and has contribution to climate change. Synthetic Aperture Radar (SAR) is one of remote sensing technology which is more efcient than optical remote sensing. Its ability to penetrate cloud makes it useful to monitor tropical environment. This research is conducted in a tropical peatland in Siak Regency, Riau Province. This research was conducted to identify tropical peatland in Siak Regency using...

Biodiversity 

Doi:10.4308/hjb.26.2.63

Cynomolgus macaques (Macaca fascicularis) are an established animal model of asthma, which exhibit different responses to allergen exposure that are clinically relevant. The chemokine ligand gene (CCL7) encodes Monocyte Chemotactic Protein-3, which has an important role in asthma pathogenesis. While CCL7 polymorphism in humans is associated with asthma phenotype, very little is known about CCL7 in nonhuman primate models of respiratory disease. The objective of this study was to isolate and char...

Molecular Biology  Biochemistry  Biodiversity 

Doi:10.4308/hjb.26.3.129