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Optimization of extracellular mannanase production from Penicillium oxalicum KUB-SN2-1 and application for hydrolysis property
Sudathip Titapoka Chantorn,Katesarin Buengsrisawat,Apinya Pokaseam,Tasanee Sombat
Songklanakarin Journal of Science and Technology , 2013,
Abstract: Effects of media composition, and physical properties on the production of crude mannanase by Penicillium oxalicumKUB-SN2-1 were investigated. P.oxalicum KUB-SN2-1 was propagated in a shaking incubator at 30°C with rotation speed of200 rpm of 7 days. The specific activity obtained during growth on robusta coffee residues (RCR) of 16.21 U/mg protein wasmuch higher than other carbon sources tested. For nitrogen sources, yeast extract (0.11 U/mg protein) and ammonium nitrate(0.09 U/mg protein) showed maximum specific activity. Hence, guar gum was the best inducer for producing mannanase (14U/mg protein). For evaluating the optimal concentration, the result showed that 1% guar gum, 0.5% yeast extract, 0.25%ammonium nitrate, and 0.25% RCR were the suitable sources of inducer, organic nitrogen, inorganic nitrogen, and carbon,respectively. Modified medium with initial culture pH of 5.0 at 30°C was optimum for mannanase production (53.77 U/ml for3 day). Reducing sugars were analyzed by dinitrosalicylic acid methods. The highest reducing sugar of 7517.82 g/mlwas obtained from copra meal hydrolysate after 30 h.
Cow manure as a lignocellulosic substrate for fungal cellulase expression and bioethanol production
Hongxing Li,Lin Zhou,Qin Yan,Xinli Liu,Yanan Wang,Yinbo Qu,Zhigang Li,Zhonghai Li
- , 2018, DOI: 10.1186/s13568-018-0720-2
Abstract: Images for corn silage diets and cow manure and anaerobically digested cow manure. Corn silage diets (a), the coarse milled corn silage diets supplemented with additional protein feed (b), solid cow manure materials after removing the liquid part by mechanical packing (c), anaerobically digested cow manure obtained from physical separation of slurry after anaerobic digestion process for biogas production (d
Pilot-scale production and optimizing of cellulolytic Penicillium oxalicum for controlling of mango malformation  [PDF]
Wafaa M. Haggag, Mostafa Abo El Soud
Agricultural Sciences (AS) , 2013, DOI: 10.4236/as.2013.44024
Abstract:

Penicillium oxalicum Curie et Thom isolated from blossom of mango (var. Saddekia) was evaluated against mango malformation incited by Fu- sarium subglutinis. Both in vitro and in vivo conditions. The mechanism of this isolate for controlling Fusarium was the reduction of growth development. The Penicillium oxalicum isolate produced extracellular cellulolytic enzymes (exo-glucanase or cellobiohydrolases and endo-glucanase, that possibly related to the biocontrol process. Optimize and fermentation conditions (growth period, carbon and nitrogen sources) for spores and cellulolytic enzymes production were determined. Liquid formulation containing (6 × 106) with sodium alginate (0.5%) and Tween 80 (0.01%)

Study of the interactions between Penicillium oxalicum Currie & Thom and Alternaria alternata (Fr.) Keissler
Sempere, F.;Santamarina, M.P;
Brazilian Journal of Microbiology , 2010, DOI: 10.1590/S1517-83822010005000003
Abstract: the aim of this research was the analysis of the possible antagonistic effect of penicillium oxalicum over the pathogen rice fungus a. alternata under different conditions of temperature, water activity and culture media. the macroscopic study of the dual growth revealed that according to the index of dominance p. oxalicum was more competitive that a. alternata at 25oc whereas at 15oc was this species. microscopic analysis showed that p. oxalicum was a mycoparasite of a. alternata at all conditions tested. the antagonist penetrated into a. alternata and disintegrated its conidiophores and conidia. the results suggests that p. oxalicum may be a possible biological control agent of the rice pathogens in a future.
Enhanced production of chitin deacetylase by Penicillium oxalicum SAEM-51 through response surface optimization of fermentation conditions
Nidhi Pareek,Sanjoy Ghosh,R. P. Singh,V. Vivekanand
3 Biotech , 2013, DOI: 10.1007/s13205-013-0118-4
Abstract: Optimization of the fermentation conditions for chitin deacetylase (CDA) production by Penicillium oxalicum SAEM-51 was undertaken in the present study using central composite design (CCD) under submerged condition. CDA is widely employed for bio-catalytic conversion of chitin to chitosan. Chitosan is a biopolymer with immense commercial potential in diverse industrial sectors, viz. pharmaceutics, food, agriculture, water treatment, etc. CDA production was significantly affected by all the variables studied, viz. pH, temperature, inoculum age and size. The optimal conditions that stimulating maximal CDA production were found to be: pH, 7.9; temperature, 28 °C; inoculum age, 90 h, and 11 % inoculum size. Under these optimized conditions, the actual maximal CDA production was 623.57 ± 8.2 Ul 1, which was in good agreement with the values predicted by the quadratic model (648.24 Ul 1), confirming the validity of the model. Optimization of fermentation conditions through CCD had resulted into 1.4-fold enhancement in CDA productivity (Q p = 4.3264 Ul 1 h 1). Results of these experiments indicated that response surface methodology was proved to be a promising method for optimization of CDA production.
溶磷真菌固体发酵菌肥对玉米生长及根际细菌群落结构的影响
孙敏,徐阳春,戴乐天,李真,王世梅,王文超,陈莎莎
农业环境科学学报 , 2018, DOI: 10.11654/jaes.2017-1629
Abstract: 溶磷微生物可将土壤中难溶性磷转化为有效态磷，施用溶磷菌肥是提高土壤磷素利用率的有效措施。选取溶磷效果较好的草酸青霉（Penicillium oxalicum）NJDL-03和黑曲霉（Aspergillus niger）NJDL-12两株真菌进行土培实验，探究了溶磷真菌在石灰性土壤中的溶磷效果，揭示了经固体发酵获得的溶磷菌肥对玉米生长及根际细菌群落结构的影响。结果表明：真菌NJDL-03和NJDL-12比细菌San8溶磷效果更强，盆栽试验中NJDL-03、NJDL-12及San8菌肥处理的土壤速效磷含量分别较CK提高了4.36、5.03、0.71倍，且NJDL-03、NJDL-12、San8及Substrate等处理的玉米植株干重分别较对照增加了67.14%、67.70%、33.96%、24.04%。各处理土壤中细菌厚壁菌门（Firmicutes）的丰度较CK显著降低，真菌菌肥处理后在细菌属水平上鞘氨醇单胞菌属（Sphingomonas）、分枝杆菌属（Mycobacterium）、小囊菌属（Plesiocystis）及嗜盐杆菌属（Halotalea）等的丰度相对于CK均有显著增加，而赖氨酸芽孢杆菌属（Lysinibacillus）及溶杆菌属（Lysobacter）丰度则较CK显著降低。生物菌肥处理提高土壤速效磷含量，影响了根际土壤细菌群落结构，并促进玉米植株生长
Cloning and expression of a novel α-1,3-arabinofuranosidase from Penicillium oxalicum sp. 68
Feng Luo,Han Zhang,Jiaqi Liu,Weiyang Wang,Xuecui Yan,Yanbo Hu,Yingying Cui
- , 2018, DOI: 10.1186/s13568-018-0577-4
Abstract: Summary of purification of recombinant PoAbf62
Solid-State Fermentation of Carrot Pomace for the Production of Inulinase by Penicillium oxalicum BGPUP-4
Chauhan, Kanika,Larroche, Christian,Pandey, Ashok,Singh, Jagroop,Singh, Ram Sarup
- , 2018, DOI: 10.1713/ftb.56.01.18.5411
Abstract: Sa？etak Inulinases are an important class of industrial enzymes which are used for the production of high-fructose syrup and fructooligosaccharides. Inulin, a polyfructan, is generally employed for the production of inulinase, which is a very expensive substrate. A number of agroindustrial residues have been used for cost-effective production of inulinases. In the present study, carrot pomace was selected as a substrate for the production of inulinase by Penicillium oxalicum BGPUP-4 in solid-state fermentation. Carrot pomace is one of the good substrates for bioprocesses, because it is rich in soluble and insoluble carbohydrates. A central composite rotatable design (CCRD) used in response surface methodology was employed for the optimal production of inulinase from carrot pomace. Using CCRD, 15 runs were practiced to optimize the range of three independent variables: moisture content (70-90 %), incubation time (4-6 days) and pH (5.0-7.0) for inulinase production. Carrot pomace supplemented with 0.5 % inulin as an inducer, 0.2 % NH4H2PO4, 0.2 % NaNO3, 0.2 % KH2PO4, 0.05 % MgSO4·7H2O and 0.001 % FeSO4·7H2O was used for the production of inulinase in solid-state fermentation at 30 °C. Inulinase production (322.10 IU per g of dry substrate) was obtained under the optimized conditions, i.e. moisture content of 90 %, incubation time 4 days and pH=7.0. The corresponding inulinase/invertase (I/S) ratio (3.38) was also high, which indicates the inulolytic nature of the enzyme. Multiple correlation coefficients R for inulinase production and I/S ratio were 0.9995 and 0.9947, respectively. The R value very close to one indicates an excellent correlation between experimental and predicted results
Study on Producing Mycelium Pellets of Penicillium oxalicum in An Airlift ReActor
应用气升式反应器培养草酸青霉菌菌丝球的研究
YUAN Li-Mei,ZHANG Shu-Jun YANG Min YANG Qing-Xiang ZHANG Li-Ping,
袁丽梅,张书军,杨敏,杨清香,章丽萍
微生物学报 , 2004,
Abstract: 应用0.5L气升式反应器成功实现了对高效染料吸附丝状真菌——草酸青霉菌(Penicillium oxalicum)的颗粒化，初步解决了菌体与培养液分离的问题。以OD600为0.23的孢子悬浊液接种时，菌丝成球的最佳接种量为2.5%，最佳通气量为0.5L/min。菌球直径及沉降速度随时间均呈线性增长。在最佳接种量（2.5%）和最佳通气量（0.5L/min）下，30℃培养72h后，菌球的直径和沉降速度分别为(4.0±0.8)mm和(14±3)mm/s。
Decolorization Ability and Microcosmic Biosorption Process of Reactive Dyes with Fungus Penicillium oxalicum
草酸青霉Penicillium oxalicum菌体吸附活性染料的性能及微观过程
ZHENG Wen-chai,XIN Bao-ping,GAN Ya-ling,LI Chang-ping,
郑文钗,辛宝平,甘雅玲,李长平
过程工程学报 , 2007,
Abstract: 研究了草酸青霉(Penicillium oxalicum)在模拟染料废水中以边生长边吸附的方式对活性翠蓝KN-G, M-GB, K-GL,活性黑K-BR、活性艳蓝M-BR、活性红紫K-3R和活性深蓝K-R 7种水溶性活性染料的脱色性能及吸附过程. 结果显示,生长菌体对7种活性染料具有良好的脱色性能,染料初始浓度为200 mg/L时,平均脱色率可达93.0%;染料初始浓度为400 mg/L时,活性翠蓝KN-G和M-GB的脱色率仍达到了99.7%和99.9%. 上清液的紫外光谱图及染料分子中铜离子浓度的检测结果表明,染料通过吸附方式从废水中去除. 通过SEM, TEM观察发现,生长菌体在吸附过程中,菌丝发生肿胀膨大,细胞壁发生结构重组,厚度增加10～15倍. 细胞壁的结构变化是生长菌体吸附染料的重要机制,为染料吸附提供位点和进入细胞内部的通道.
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