oalib

OALib Journal期刊

ISSN: 2333-9721

费用：99美元

投稿

时间不限

( 2026 )

( 2025 )

( 2024 )

( 2023 )

自定义范围…

匹配条件： “Ca2 -Mg2 -ATPase” ，找到相关结果约1000条。
列表显示的所有文章，均可免费获取
第1页/共1000条
每页显示 条
(Ca2+-Mg2+)ATPase in Schistosoma mansoni: evidence for heterogeneity and resistance to praziquantel
Cunha VMN,No？l F
Memórias do Instituto Oswaldo Cruz , 1998,
Abstract:
Effect of untreated pharmaceutical plant effluent on cardiac Na+-K+- ATPase and Ca2+-Mg2+-ATPase activities in mice (Mus Musculus)
A.O. Abdulkareem,L.A. Olatunji,O.O. Akinbobola,S.A. Busari,T.F. Olafimihan
- , 2019, DOI: 10.1016/j.toxrep.2019.05.002
Abstract: Cardiovascular diseases are major causes of non-communicable diseases (NCDs)-related throughout the world. Water pollution has been linked with the high global NCD burden but no report exists on the cardiotoxicity of untreated or poorly treated pharmaceutical effluent, despite its indiscriminate discharge into the aquatic environment in Nigeria, as in many other locations of the world. Thus, this study investigated the cardiotoxic effect of oral exposure to pharmaceutical effluent in mice. Thirty (30) male mice (Mus musculus) were randomly divided into 6 groups. Group A (control) received 0.2？ml distilled water, while groups B-F were treated with 0.2？ml 2.5%, 5.0%, 10.0%, 20.0% and 40% concentrations (v/v, effluent/distilled water) of the effluent respectively, for 28 days. Significant reductions (p<0.05) in heart weight and cardiac weight index were observed in the groups treated with 5%, 10%, 20% and 40% concentrations of the effluent, without significant change in body weight. Similarly, 28 day administration of the effluent showed significant decrease in cardiac Na+-K+-ATPase activity (p<0.05) at concentrations 10% and above, in a concentration dependent manner. However, there was insignificant decrease in cardiac Ca2+-Mg2+-ATPase activity of the exposed mice, when compared with the control group. This study provides novel information on the cardiotoxic effects of oral exposure to untreated pharmaceutical effluent, showing reduced Na+-K+-ATPase activity and decreseased myocardial atrophy. Therefore, drinking water contaminated with pharmaceutical effluent may promote the incidence of cardiovascular diseases. Further studies on the exact mechanistic routes of the induced cardiotoxicity are recommended
Isolation of Mung Bean Plasma Membrane Vesicles and the Analyses of Hydrolysis Activity of PM H+-ATPase
绿豆下胚轴质膜微囊的提取和H+-ATPase 活性研究
WEN Bin BIN Jin-Hua PAN Rui-Chi WANG Xiao-Jing,
文彬,宾金华,潘瑞炽,王小菁
植物学报 , 2004,
Abstract: The aqueous two-phase partition method was used to isolate and purify plasma membrane (PM) vesicles from mung bean hypocotyls. The PM vesicles were purified well when the proportion of the weight of hypocotyls and two-phase partition reached 32:8. Bovine serum albumin in homogenization medium influenced the latency activity of the PM H+-ATPase. The enzyme hydrolysis activity was Mg2+ dependent and was strongly stimulated by Ca2+. Fusicoccin (FC), an activator of PM H+-ATPase, stimulated the enzyme activity with the maximal increase of 72% in vivo and of 30% in vitro as compared with control.
绿豆下胚轴质膜微囊的提取和H+-ATPase活性研究
文彬,宾金华,潘瑞炽,王小菁
植物学报 , 2004,
Abstract: ？以两相法提取纯化绿豆下胚轴质膜微囊，材料与两相体系重量之比为32∶8时，一次洗膜就可以得到纯度较高的质膜微囊。提取缓冲液中牛血清白蛋白的浓度对质膜H+-ATPase的潜在活性有影响。质膜H+-ATPase水解活性依赖于Mg2+，Ca2+对酶活性有明显的促进作用。壳梭孢素（fusicoccin,FC）对酶有明显的刺激作用，活体条件最大刺激达到72％，而离体条件下刺激为30％。
肌浆网钙泵蛋白的结构和功能及其蛋白单体的特性
陈曦？
生物化学与生物物理进展 , 1991,
Abstract: 肌浆网是细胞钙平衡的主要调节单位之一,是胞浆钙离子浓度异常的重要研究对象。肌浆网结构组成简单,便于分离纯化,是理想的生物膜研究模型。本文介绍了肌浆网钙泵蛋白的分子结构、钙转运分子机制和钙泵蛋白的聚集状态及磷脂结构与钙转运的关系。
Physiological effects of five different marine natural organic matters (NOMs) and three different metals (Cu, Pb, Zn) on early life stages of the blue mussel (Mytilus galloprovincialis)
Adalto Bianchini,Chris M. Wood,Lygia Sega Nogueira,Marianna Basso Jorge,Rachael L. Diamond,Scott Smith
- , 2017, DOI: 10.7717/peerj.3141
Abstract: Metals are present in aquatic environments as a result of natural and anthropogenic inputs, and may induce toxicity to organisms. One of the main factors that influence this toxicity in fresh water is natural organic matter (NOM) but all NOMs are not the same in this regard. In sea water, possible protection by marine NOMs is not well understood. Thus, our study isolated marine NOMs by solid-phase extraction from five different sites and characterized them by excitation-emission fluorescence analysis—one inshore (terrigenous origin), two offshore (autochthonous origin), and two intermediate in composition (indicative of a mixed origin). The physiological effects of these five NOMS alone (at 8 mg/L), of three metals alone (copper, lead and zinc at 6 μg Cu/L, 20 μg Pb/L, and 25 μg Zn/L respectively), and of each metal in combination with each NOM, were evaluated in 48-h exposures of mussel larvae. Endpoints were whole body Ca2++Mg2+-ATPase activity, carbonic anhydrase activity and lipid peroxidation. By themselves, NOMs increased lipid peroxidation, Ca2++Mg2+-ATPase, and/or carbonic anhydrase activities (significant in seven of 15 NOM-endpoint combinations), whereas metals by themselves did not affect the first two endpoints, but Cu and Pb increased carbonic anhydrase activities. In combination, the effects of NOMs predominated, with the metal exerting no additional effect in 33 out of 45 combinations. While NOM effects varied amongst different isolates, there was no clear pattern with respect to optical or chemical properties. When NOMs were treated as a single source by data averaging, NOM had no effect on Ca2++Mg2+-ATPase activity but markedly stimulated carbonic anhydrase activity and lipid peroxidation, and there were no additional effects of any metal. Our results indicate that marine NOMs may have direct effects on this model marine organism, as well as protective effects against metal toxicity, and the quality of marine NOMs may be an important factor in these actions
Effects of Shenfu Injection on prostacyclin, thromboxane A2 and activities of ATPases in rats exposed to hepatic ischemia-reperfusion injury
Song-lin PENG,Chao-liu DAI
Zhong Xi Yi Jie He Xue Bao , 2007,
Abstract: Objective: To explore the effects of Shenfu Injection on prostacyclin, thromboxane A2 and the activities of ATPases in rats exposed to hepatic ischemia-reperfusion injury.Methods: Twenty-four male Wistar rats weighing 200-250 g were randomly divided into two groups: Shenfu Injection (SF)-treated group (rats were treated with Shenfu Injection of 10 ml/kg through intraperitoneal injection) and untreated group (rats were administered with normal saline at the same dose and served as a control group). Hepatic ischemia was caused by Pringle's maneuver and lasted for fifteen minutes, and then one-hour or three-hour reperfusion was performed. Venous blood samples for the measurement of thromboxane B2 (TXB2) and 6-keto-prostaglandin F1α (6-keto-PGF1α) were collected three hours after reperfusion. Liver tissue samples were collected one hour or three hours after reperfusion for the measurement of Na+-K+-ATPase and Ca2+-Mg2+-ATPase and for morphological studies.Results: Plasma TXB2 was lower in the SF-treated group than that in the untreated group after three-hour reperfusion (P＞0.05), while 6-keto-PGF1α was higher in the SF-treated group than that in the untreated group (P＞0.05). The ratio of TXB2 and 6-keto-PGF1α was significantly lower in the SF-treated group than that in the untreated group (P＜0.05). The activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in the SF-treated group were improved obviously. A three-hour reperfusion after fifteen-minute ischemia caused important hepatic histological alterations. Marked structural abnormalities were observed in the untreated group, such as massive hepatocyte swelling, necrosis, mitochondria edema and vacuolar changes. In the SF-treated group, hepatic tissue injury was reduced significantly.Conclusion: Shenfu Injection protects hepatic tissue from ischemia-reperfusion injury, and such protective effects are achieved by decreasing the ratio of thromboxane A2 and prostacyclin, and increasing the activities of Na+-K+-ATPase and Ca2+-Mg2+- ATPase.
瑞香狼毒对东亚飞蝗作用机理的初步研究
Studies on the mechanism of Stellera chamaejasme L. to Locusta migratoria manilensis (Meyen)
陈琼,高甜甜,李波,查艳梅,陶科,金洪,侯太平
- , 2017,
Abstract: 利用硅胶柱分离瑞香狼毒乙酸乙酯萃取物得到I、II、III段粗分物，并测定对东亚飞蝗毒杀活性最高的I段粗分物对东亚飞蝗的致死性及对其体内Na+-K+-ATPase、Ca2+/Mg2+-ATPase、乙酰胆碱酯酶的抑制活性. 研究表明：瑞香狼毒I段粗分物对东亚飞蝗具有较强的毒杀作用，在药物浓度为10mg/mL、72h对东亚飞蝗的校正死亡率为92.5%；同时，毒杀作用具有浓度和时间依赖性；而且瑞香狼毒乙酸乙酯萃取物I段粗分物对上述3种酶均有一定的抑制作用，且对Na+-K+-ATPase的抑制作用最强，在药物浓度为5mg/mL、96h对Na+-K+-ATPase的抑制率为40.35%；分析发现瑞香狼毒活性成分对东亚飞蝗的毒杀作用与对Na+-K+-ATPase的抑制作用之间具有显著相关性，据此推测，Na+-K+-ATPase可能是瑞香狼毒活性成分的主要作用靶标.
Using silica gel column to separate the ethyl acetate extract of Stellera chamaejasme L. we can get I, II, III sections. The activity of Na+-K+-ATPase、Ca2+/Mg2+-ATPase、acetylcholinesterase in insects were determined in vivo. The results showed that I section from Stellera chamaejasme L. had the strongest poison effect on Locusta migratoria manilensis (Meyen). Under the concentration of 10 mg/mL, the corrected mortality was 92.5% in 72 hour. And the poison effect there was persistence. The I section from Stellera chamaejasme L. also had certain inhibition on activity of Na+-K+-ATPase、Ca2+/Mg2+-ATPase、acetylcholinesterase. The inhibiton rate of Na+-K+-ATPase was the highest among the three kinds of enzymes. There was a well correlation between the toxic action of the active ingredients from Stellera chamaejasme L. to Locusta migratoria manilensis (Meyen) and the inhibition on activity of Na+-K+-ATPase in Locusta migratoria manilensis (Meyen). Na+-K+-ATPase is likely the target of the active ingredients from Stellera chamaejasme L. against Locusta migratoria manilensis (Meyen)
Thyroid Hormone Homeostasis in Adult Mammalian Brain: A Novel Mechanism for Functional Preservation of Cerebral T3 Content During Initial Peripheral Hypothyroidism
Samita Kundu,Arun K Ray
Al Ameen Journal of Medical Sciences , 2010,
Abstract: brain is well known. But the action of THs in the adult brain was not widely a focus of study by endocrinologists based on lack of increased energy metabolism and oxygen consumption with changing thyroid status and thus not widely acknowledged. Extensive research has, however, revealed interesting findings like sequestration of T3, possible release of T3 as a neurotransmitter in nerve terminals, identification of specific membrane binding sites of T3 in the synaptosomal fraction of adult rat brain and many non-genomic neurotransmitter-like actions of TH in the adult mammalian brain. Most importantly, thyroid dysfunction is associated with significant disruption of psychobehavioural system in the adult, which can however be reversed with therapeutic hormonal intervention. A complex regulatory network involving transfer of TH through the brain barriers, interactions between neurons and glial cells, and deiodinase expression works synchronously to deliver the appropriate amount of T3 to the neurons. Despite peripheral hypo- or hyper-thyroidism, brain can maintain a normal level of TH up to certain duration. Thus, presence of a novel homeostatic mechanism in the adult mammalian brain (‘central homeostasis for thyroid hormone’) to defend the adverse neuropsychological manifestations commonly associated with peripheral hypothyroidism has been known for a long time. Unfortunately, the exact time course and the mechanism of such central homeostasis were not determined, till we made a pioneering attempt to evaluate the same. The entire phenomenon appeared to be coupled with nuclear mediated genomic processes like mRNA and protein synthesis. Moreover, the effects of THs on some key enzymes and ions related to neurotransmission during the start and end days of this central homeostatic phenomenon point towards a dependency of the enzymes on TH and an involvement of TH in the neurobiochemical events
Effects of aqueous extract of Hibiscus sabdariffa on renal Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in Wistar rats
Lawrence A. Olatunji
Zhong Xi Yi Jie He Xue Bao , 2012,
Abstract: OBJECTIVE: To investigate the effects of oral administration of aqueous extract of Hibiscus sabdariffa on renal Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in rats.METHODS: The 25 and 50 mg/(kg·d) of aqueous extracts of H. sabdariffa were respectively given to rats in the experimental groups for 28 d, and rats in the control group received an appropriate volume of distilled water as vehicle. Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in the kidney were assayed by spectrophotometric method.RESULTS: Administrations of 25 and 50 mg/(kg·d) of aqueous extract of H. sabdariffa significantly decreased the Ca2+-Mg2+-ATPase activity in the kidney of rats (P<0.05). However, the renal Na+-K+-ATPase activity of the experimental rats was not affected by either dose of the extract. And the plasma Na+, K+ and Ca2+ levels of the experimental rats had no significant changes. Administration of either dose of the extract did not result in any significant changes in body and kidney weights, the concentrations of plasma albumin and total protein, and alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase activities. However, concentrations of creatinine and urea were significantly reduced by 50 mg/kg of the extract (P<0.05).CONCLUSION: The present study indicates that oral administration of aqueous extract of H. sabdariffa may preserve the renal function despite a decreased renal Ca2+-Mg2+-ATPase activity.
第1页/共1000条
每页显示 条


Home
Copyright © 2008-2020 Open Access Library. All rights reserved.