Groundwater remains a critical resource for rural communities in South Africa, yet its quality is questionable due to geogenic processes and anthropogenic activities. This research evaluated groundwater quality through hydrochemical and microbial analysis of 20 borehole samples used domestically in the rural area of Zebediela subregion, Limpopo Province, South Africa. Parameters analysed included major ions, trace metals and faecal indicator bacteria, with data interpreted using multivariate analyses including co-occurrence matrices, Pearson correlation heatmaps and Principal Component Analysis. Nitrate, sulphate, phosphate, chloride and fluoride were within permissible limits, but COD and BOD were slightly above recommended levels, indicating moderate ion concentrations. Microbial contamination was detected in 80% of the samples (including Heterotrophic, Escherichia coli, coliform, Salmonella species, Enterobacteriaceae, Bacillus cereus, Staphylococcus aureus and Enterococci), with Heterotrophic bacteria present in most samples, indicating emerging contamination. PCA identified mineral dissolution, evaporative concentration, organic pollution and redox-mediated metal mobilization as the dominant processes shaping groundwater chemistry in Zebediela subregion. Therefore, regular monitoring, improved sanitation infrastructure and protection of borehole surroundings are recommended to manage water security in the Zebediela sub-region.
References
[1]
Baddianaah, I., Dongzagla, A. and Salifu, S.N. (2024) Navigating Access to Safe Water by Rural Households in Sub-Saharan Africa: Insights from North-Western Ghana. Sustainable Environment, 10, Article ID: 2303803. https://doi.org/10.1080/27658511.2024.2303803
[2]
Ejiohuo, O., Onyeaka, H., Akinsemolu, A., Nwabor, O.F., Siyanbola, K.F., Tamasiga, P., et al. (2025) Ensuring Water Purity: Mitigating Environmental Risks and Safeguarding Human Health. Water Biology and Security, 4, Article ID: 100341. https://doi.org/10.1016/j.watbs.2024.100341
[3]
Tenebe, I.T., Babatunde, E.O., Ogarekpe, N.M., Emakhu, J., Etu, E., Edo, O.C., et al. (2024) Detection and Measurement of Bacterial Contaminants in Stored River Water Consumed in Ekpoma. Water, 16, Article No. 2696. https://doi.org/10.3390/w16182696
[4]
Tshona, S.S., Lungisa, S. and Mgweba, L. (2025) Thirsting for Solutions: Unpacking Inadequate Water Provision in Rural Communities. Africa’s Public Service Delivery and Performance Review, 13, a873. https://doi.org/10.4102/apsdpr.v13i1.873
[5]
Voudouris, K., Valipour, M., Kaiafa, A., Zheng, X.Y., Kumar, R., Zanier, K., et al. (2018) Evolution of Water Wells Focusing on Balkan and Asian Civilizations. Water Supply, 19, 347-364. https://doi.org/10.2166/ws.2018.114
[6]
Promilton, A.A.A., Ravindran, A.A., Pitchaimani, V.S., Kingston, J.V. and Karuppannan, S. (2025) Comprehensive Hydrogeochemical Characterization and Seasonal Water Quality Index Analysis for Sustainable Groundwater Management in Valliyur Region, Southern Tamil Nadu, India. Scientific Reports, 15, Article No. 33251. https://doi.org/10.1038/s41598-025-18285-3
[7]
Akhtar, N., Syakir Ishak, M.I., Bhawani, S.A. and Umar, K. (2021) Various Natural and Anthropogenic Factors Responsible for Water Quality Degradation: A Review. Water, 13, Article No. 2660. https://doi.org/10.3390/w13192660
[8]
Ravi, M., et al. (2025) Hydrogeochemical Evaluation and Groundwater Quality Assessment in Madurai South Taluk, Tamil Nadu, India. Journal of Environmental Studies, 39, 25-44. https://doi.org/10.21608/jesj.2025.390385.1130
[9]
Egbueri, J.C., Agbasi, J.C., Ayejoto, D.A., Khan, M.I. and Khan, M.Y.A. (2023) Extent of Anthropogenic Influence on Groundwater Quality and Human Health-Related Risks: An Integrated Assessment Based on Selected Physicochemical Characteristics. Geocarto International, 38, Article ID:2210100. https://doi.org/10.1080/10106049.2023.2210100
[10]
Shokoohi, E. and Moyo, N. (2025) Groundwater Quality in a Rural and Urbanized Region in Limpopo Province, South Africa. Environments, 12, Article No. 174. https://doi.org/10.3390/environments12060174
[11]
Ndlangamandla, L., Sukdeo, N. and Mukwakungu, S.C. (2024) Water Quality Improvement for Food and Beverage Industry vs. SANS:241. Proceedings of the International Conference on Industrial Engineering and Operations Management, Tokyo, 10-12 September 2024, 1028-1041. https://doi.org/10.46254/ap05.20240242
[12]
Florez-Peñaloza, J.R., Mahlknecht, J., Escolero, O., Morales-Casique, E., Montaño-Caro, J.C., Blanco-Gaona, S., et al. (2025) Hydrogeochemical Evolution of a Semiarid Endorheic Basin, with Intense Agricultural and Livestock Activities. Journal of Hydrology, 657, Article ID:133093. https://doi.org/10.1016/j.jhydrol.2025.133093
[13]
Hamma, B., Bekkouche, M.F., Bouaicha, F., Elnagdy, K.A., Alzaed, A., Barkat, A., et al. (2025) Hydrogeochemical Assessment of Groundwater for Agricultural Suitability in the Ksour Mountains, Algeria. Scientific Reports, 15, Article No. 36441. https://doi.org/10.1038/s41598-025-20352-8
[14]
Sambo, T.D., Gololo, S.S. and Seeletse, S.M. (2025) Borehole Water Quality and Health Risks in Rural Communities: A Consumer Perceptive Analysis. International Journal of Business Ecosystem & Strategy, 7, 420-429. https://doi.org/10.36096/ijbes.v7i3.818
[15]
Mosase, E. and Ahiablame, L. (2018) Rainfall and Temperature in the Limpopo River Basin, Southern Africa: Means, Variations, and Trends from 1979 to 2013. Water, 10, Article No. 364. https://doi.org/10.3390/w10040364
[16]
World Health Organization (2022) Guidelines for Drinking-Water Quality, Fourth Edition Incorporating the First and Second Addenda. World Health Organization.
[17]
Mabe, C.J., Molefe, D.M. and Gololo, S.S. (2024) Investigating the Presence and Levels of Some Selected Chemical Parameters in Borehole Water of Ga-Matlala in Limpopo Province, South Africa: Determining the Potential Risks. Environmental Health Insights, 18, 1-2. https://doi.org/10.1177/11786302241297492
[18]
Custodio, M., De la Cruz, H., Huarcaya, J. and Huanay, Y. (2025) Tap Water Quality from Surface and Groundwater Sources in Rural and Urban Communities Evaluated in Contrasting Climatic Seasons and Its Implications for Public Health. Journal of Water and Health, 23, 1196-1214. https://doi.org/10.2166/wh.2025.045
[19]
Maluleke, T.P., Dube, S., Sunkari, E.D. and Ambushe, A.A. (2025) Assessment of Borehole Water Quality in Nwadzekudzeku Village, Giyani, Limpopo Province, South Africa: Implication for Potential Human Health Risks. Journal of Trace Elements and Minerals, 11, Article ID: 100206. https://doi.org/10.1016/j.jtemin.2024.100206
[20]
Heaviside, C., Witham, C. and Vardoulakis, S. (2021) Potential Health Impacts from Sulphur Dioxide and Sulphate Exposure in the UK Resulting from an Icelandic Effusive Volcanic Eruption. Science of the Total Environment, 774, Article ID: 145549. https://doi.org/10.1016/j.scitotenv.2021.145549
[21]
Fuge, R. (2019) Fluorine in the Environment, a Review of Its Sources and Geochemistry. Applied Geochemistry, 100, 393-406. https://doi.org/10.1016/j.apgeochem.2018.12.016
[22]
Guissouma, W., Hakami, O., Al-Rajab, A.J. and Tarhouni, J. (2017) Risk Assessment of Fluoride Exposure in Drinking Water of Tunisia. Chemosphere, 177, 102-108. https://doi.org/10.1016/j.chemosphere.2017.03.011
[23]
Stadler, S., Talma, A., Tredoux, G. and Wrabel, J. (2012) Identification of Sources and Infiltration Regimes of Nitrate in the Semi-Arid Kalahari: Regional Differences and Implications for Groundwater Management. Water SA, 38, 213-224. https://doi.org/10.4314/wsa.v38i2.6
[24]
Fossen Johnson, S. (2019) Methemoglobinemia: Infants at Risk. Current Problems in Pediatric and Adolescent Health Care, 49, 57-67. https://doi.org/10.1016/j.cppeds.2019.03.002
[25]
Ma, L., Hu, L., Feng, X. and Wang, S. (2018) Nitrate and Nitrite in Health and Disease. Aging and disease, 9, Article No. 938. https://doi.org/10.14336/ad.2017.1207
[26]
Lacalamita, D., Mongioví, C. and Crini, G. (2024) Chemical Oxygen Demand and Biochemical Oxygen Demand Analysis of Discharge Waters from Laundry Industry: Monitoring, Temporal Variability, and Biodegradability. Frontiers in Environmental Science, 12, Article ID:1387041. https://doi.org/10.3389/fenvs.2024.1387041
[27]
Adeyemi, A.I. (2020) Bacteriological and Physicochemical Quality of Borehole Water Used for Drinking at Olusegun Agagu University of Science and Technology, Okitipupa, Nigeria. International Journal of Environment, Agriculture and Biotechnology, 5, 890-897. https://doi.org/10.22161/ijeab.54.7
[28]
Hama Aziz, K.H., Mustafa, F.S., Omer, K.M., Hama, S., Hamarawf, R.F. and Rahman, K.O. (2023) Heavy Metal Pollution in the Aquatic Environment: Efficient and Low-Cost Removal Approaches to Eliminate Their Toxicity: A Review. RSC Advances, 13, 17595-17610. https://doi.org/10.1039/d3ra00723e
[29]
Acosta, J.A., Jansen, B., Kalbitz, K., Faz, A. and Martínez-Martínez, S. (2011) Salinity Increases Mobility of Heavy Metals in Soils. Chemosphere, 85, 1318-1324. https://doi.org/10.1016/j.chemosphere.2011.07.046
[30]
Deng, H., Tu, Y., Wang, H., Wang, Z., Li, Y., Chai, L., et al. (2022) Environmental Behavior, Human Health Effect, and Pollution Control of Heavy Metal(loid)s toward Full Life Cycle Processes. Eco-Environment & Health, 1, 229-243. https://doi.org/10.1016/j.eehl.2022.11.003
[31]
Liu, W., Qin, D., Yang, Y. and Guo, G. (2023) Enrichment of Manganese at Low Background Level Groundwater Systems: A Study of Groundwater from Quaternary Porous Aquifers in Changping Region, Beijing, China. Water, 15, Article No. 1537. https://doi.org/10.3390/w15081537
[32]
DeVore, C.L., Rodriguez-Freire, L., Villa, N., Soleimanifar, M., Gonzalez-Estrella, J., Ali, A.M.S., et al. (2022) Mobilization of As, Fe, and Mn from Contaminated Sediment in Aerobic and Anaerobic Conditions: Chemical or Microbiological Triggers? ACS Earth and Space Chemistry, 6, 1644-1654. https://doi.org/10.1021/acsearthspacechem.1c00370
[33]
Odewade, L.O., Imam, A.A., Adesakin, T.A. and Odewade, J.O. (2025) Assessment of Human Faecal Contamination on Groundwater Quality and Reporting Consequent Waterborne Diseases in Funtua Metropolis, Katsina State, Nigeria. Frontiers in Water, 7, Article ID: 1561777. https://doi.org/10.3389/frwa.2025.1561777
[34]
Xiao, Q., Wang, B., Li, Z., Zhang, Z., Xie, K., Zhou, J., et al. (2024) The Assembly Process and Co-Occurrence Network of Soil Microbial Community Driven by Cadmium in Volcanic Ecosystem. Resources, Environment and Sustainability, 17, Article ID: 100164. https://doi.org/10.1016/j.resenv.2024.100164
[35]
Kristanti, R.A., Hadibarata, T., Syafrudin, M., Yılmaz, M. and Abdullah, S. (2022) Microbiological Contaminants in Drinking Water: Current Status and Challenges. Water, Air, & Soil Pollution, 233, Article No. 299. https://doi.org/10.1007/s11270-022-05698-3
[36]
Lin, L., Yang, H. and Xu, X. (2022) Effects of Water Pollution on Human Health and Disease Heterogeneity: A Review. Frontiers in Environmental Science, 10, Article ID: 880246. https://doi.org/10.3389/fenvs.2022.880246
[37]
Ben Maamar, S., Aquilina, L., Quaiser, A., Pauwels, H., Michon-Coudouel, S., Vergnaud-Ayraud, V., et al. (2015) Groundwater Isolation Governs Chemistry and Microbial Community Structure along Hydrologic Flowpaths. Frontiers in Microbiology, 6, Article No. 1457. https://doi.org/10.3389/fmicb.2015.01457
[38]
Said, I., Abd-Elgawad, A.N., Seleem, E.M., Zeid, S.A.M. and Salman, S.A. (2022) Multivariate Statistics Explaining Groundwater Chemistry, Asyut, Egypt. Environmental Monitoring and Assessment, 194, Article No. 669. https://doi.org/10.1007/s10661-022-10338-8
