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蝙蝠蛾被毛孢–红景天–灵芝复方制剂对小鼠免疫功能的影响研究
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Abstract:
为研究蝙蝠蛾被毛孢–红景天–灵芝复方制剂的免疫调节作用，以SPF级昆明雄性小鼠为模型，灌胃给予225、450、900 mg/kg BW (相当于人体推荐用量的5、10、20倍)的样品，连续干预30天。采用噻唑蓝比色法、耳肿胀法、Jerne改良玻片法等检测脾淋巴细胞增殖、迟发型变态反应(DTH)、抗体生成细胞数等免疫指标。结果表明，该制剂可显著促进小鼠脾淋巴细胞转化、增强DTH反应、提高抗体生成细胞数及血清溶血素水平，并增强单核–巨噬细胞碳廓清能力，对小鼠体重及免疫器官发育无显著影响。本研究为该复方作为免疫调节类健康食品的开发提供实验依据。
To investigate the immunomodulatory effects of a compound preparation containing Hirsutella sinensis, Rhodiola, and Ganoderma lucidum, SPF-grade male Kunming mice were used as the model. The mice were administered the sample via oral gavage at doses of 225, 450, and 900 mg/kg body weight (equivalent to 5, 10, and 20 times the recommended human dosage, respectively) for 30 consecutive days. Immune parameters, including splenic lymphocyte proliferation, delayed-type hypersensitivity (DTH) response, and antibody-producing cell count, were assessed using MTT colorimetric assay, ear swelling method, and Jerne’s modified slide method, respectively. The results demonstrated that the compound preparation significantly promoted splenic lymphocyte transformation, enhanced the DTH response, increased the number of antibody-producing cells and serum hemolysin levels, and improved the carbon clearance capacity of mononuclear-macrophages. No significant effects on body weight or immune organ development were observed in the mice. This study provides experimental evidence supporting the development of this compound preparation as a health food with immunomodulatory functions.
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