This study evaluates the effects of dietary supplementation with Mangifera indica (mango), Lawsonia inermis (henna), and Carica papaya (papaya) leaf powders on antioxidant activity, lipid metabolism, immune response, and meat quality in broiler chickens. Fresh leaves were shade-dried, ground into powder, and incorporated into feed at specific concentrations for 600-day-old chicks divided into five groups (n = 120/group). Key parameters—including blood biochemistry, antioxidant enzyme activity (MDA, SOD, GPx), lipid profiles, and immune markers—were assessed using standardized protocols. Results indicate that mixed herbal supplementation (Group E) demonstrated the highest DPPH radical scavenging activity (45.23% ± 1.48%, p < 0.0001), significantly exceeding the single-herb groups. Additionally, oxidative stress markers improved, with MDA levels lowest in Group E (1.24 ± 0.31 nmol/mL, p = 0.0103), and SOD (92.61 ± 10.08 U/mL) and GPx (90.62 ± 12.08 U/mL) activities highest in this group (p < 0.05). Lipid metabolism was optimized, showing moderate cholesterol (86.00 ± 0.78 mg/dL) and the lowest LDL levels (41.33 ± 0.44 mg/dL, p < 0.0001). Carica papaya supplementation (Group D) effectively reduced cholesterol (56.00 ± 0.43 mg/dL) and triglycerides (108.33 ± 0.66 mg/dL). Furthermore, immune responses improved, with increased IgG (4.30 ± 0.14 mg/dL), IgM (2.85 ± 0.13 mg/dL), and Newcastle disease antibody titers (7.00 ± 0.22, p < 0.0001). The combination of herbal additives also enhanced meat quality by improving water-holding capacity and reducing cooking loss. These findings suggest that dietary inclusion of these herbal leaf powders can enhance broiler health, immune function, and meat quality.
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