This study aimed to optimization of the in vitro fertilization system in C？ goat oocytes to achieve the maximum possible blastocyst development rate. In Experiment 1, we assessed the effects of IVF media on the in vitro fertilization of C？ goat oocytes. There was no significant difference in the cleavage, blastocyst, or hatching rates between TALP-Fert and BO-IVF media. Experiment 2 was performed to assess the concentration of sperm in the in vitro fertilization of C？ goat oocytes. The matured C？ goat oocytes were fertilized in BO-IVF for four sperm concentrations: 5 × 105, 1 × 106, 2 × 106 and 3 × 106 sperm/ml. The blastocyst rate of 2 × 106 sperm/ml and 3 × 106 sperm/ml groups was higher than that of 5 × 105 sperm/ml and 1 × 106 sperm/ml groups (P < 0.05). Experiment 3 was performed to assess the IVF duration on the in vitro fertilization of C？ goat oocytes. The matured C？ goat oocytes were fertilized in BO-IVF with sperm concentration of 3 × 106 sperm/ml for 18, 20, 22 and 24 h. The cleavage, blastocyst, and hatching blastocyst rates of 18 h group were lower than those of 20, 22 and 24 h groups (P < 0.05). The difference in cleavage, blastocyst and hatching blastocyst rates between 20, 22 and 24 h groups was not statistically significant (P > 0.05). In conclusion, the matured C？ goat oocytes were fertilized in BO-IVF with sperm concentration of 3 × 106 sperm/ml for 20 hours, which is suitable for the in vitro C？ goat embryo production.
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