Evaluating the Effects on Ultrasound and on Magnetic Resonance for Knee Osteoarthritis Treatment by Platelet Rich Plasma Therapy in Combination with Mesenchymal Stem Cells from Autologous Adipose Tissue
Aim: To evaluate the effects based the changes on ultrasound and on magnetic resonance for knee osteoarthritis treatment by platelet-rich plasma therapy in combination with mesenchymal stem cell from autologous adipose tissue. Objects and Methods: 30 patients, 26 females, 4 males, mean age 58.63 ± 11.11, mean disease duration 5.3 ± 4.6 years, respectively 60 knee joints were diagnosed with osteoarthritis at stage II - III according to the Kellgren and Lawrence classifications. After ultrasound and magnetic resonance imaging in the first time, all patients were injected with platelet-rich plasma combined with mesenchymal stem cells from autologous adipose tissue into both knee joints. Results: After treatment 12 months by platelet-rich plasma injection combined with mesenchymal stem cells from autologous adipose tissue: The thickness of articular cartilage on ultrasound increased significantly from 2.08 ± 0.36 mm to 2.48 ± 0.36 mm with p < 0.05. There were 52 joints with increased cartilage thickness, equivalent to 86.67%. The surface of articular cartilage on magnetic resonance in all positions of the knee joint has improved, the difference was statistically significant. Particularly, the patellar joint improved from 1.56 ± 0.25 mm to 1.64 ± 0.21 mm with p < 0.05. Conclusion: The treatment of knee osteoarthritis by platelet-rich plasma combined with mesenchymal stem cells from autologous adipose tissue was effective in improving cartilage thickness on ultrasound and magnetic resonance, thereby improving the motor function of the knee joint.
References
[1]
El-Sharkawy, H., Kantarci, A., Deady, J., et al. (2007) Platelet-Rich Plasma: Growth Factors and Pro- and Anti-Inflammatory Properties. Journal of Periodontology, 78, 661-669. https://doi.org/10.1902/jop.2007.060302
[2]
Sampson, S., Reed, M., Silvers, H., Meng, M. and Mandelbaum, B. (2010) Injection of Platelet-Rich Plasma in Patients with Primary and Secondary Knee Osteoarthritis: A Pilot Study. American Journal of Physical Medicine & Rehabilitation, 89, 961-969. https://doi.org/10.1097/PHM.0b013e3181fc7edf
[3]
Coulson-Thomas, V.J., Coulson-Thomas, Y.M., Gesteira, T.F. and Kao, W.Y. (2016) Extrinsic and Intrinsic Mechanisms by Which Mesenchymal Stem Cells Suppress the Immune System. The Ocular Surface, 14, 121-134. https://doi.org/10.1016/j.jtos.2015.11.004
[4]
Ryu, J.-S., Jung, Y.-H., Cho, M.-Y, Yeo, J.E., Choi, Y.-J., Kim, Y.I., et al. (2014) Co-Culture with Human Synovium-Derived Mesenchymal Stem Cells Inhibits Inflammatory Activity and Increases Cell Proliferation of Sodium Nitroprusside-Stimulated Chondrocytes. Biochemical and Biophysical Research Communications, 447, 715-720. https://doi.org/10.1016/j.bbrc.2014.04.077
[5]
Barry, F. and Murphy, M. (2013) Mesenchymal Stem Cells in Joint Disease and Repair. Nature Reviews Rheumatology, 9, 584-594. https://doi.org/10.1038/nrrheum.2013.109
[6]
Mamidi, M.K., Das, A.K., Zakaria, Z. and Bhonde, R. (2016) Mesenchymal Stromal Cells for Cartilage Repair in Osteoarthritis. Osteoarthritis and Cartilage, 24, 1307-1316. https://doi.org/10.1016/j.joca.2016.03.003
[7]
Caplan, A.I. (2010) Adult Mesenchymal Stem Cells for Tissue Engineering versus Regenerative Medicine. Journal of Cellular Physiology, 213, 341-347. https://doi.org/10.1002/jcp.21200
[8]
Grässel, S. and Lorenz, J. (2014) Tissue-Engineering Strategies to Repair Chondral and Osteochondral Tissue in Osteoarthritis: Use of Mesenchymal Stem Cells. Current Rheumatology Reports, 16, Article No. 452. https://doi.org/10.1007/s11926-014-0452-5
[9]
Zuk, P.A., et al. (2001) Multilineage Cells from Human Adipose Tissue: Implications for Cell-Based Therapies. Tissue Engineering, 7, 211-228.
[10]
Song, Y., et al. (2020) Mesenchymal Stem Cells in Knee Osteoarthritis Treatment: A Systematic Review and Meta-Analysis. Journal of Orthopaedic Translation, 24, 121-130. https://doi.org/10.1016/j.jot.2020.03.015
[11]
Toghraie, F., Razmkhah, M., Gholipour, M.A., et al. (2012) Scaffold-Free Adipose Derived Stem Cells (ASCs) Improve Experimentally Induced Osteoarthritis in Rabbits. Archives of Iranian Medicine, 15, 495-499.
[12]
Lu, L.-J., et al. (2016) Treatment with Human Adipose-Derived Mesenchymal Stem Cells for Knee Osteoarthritis. NCT 021626693. The 18th Congress of Asia Pacific League of Association for Rheuatology (APLAR 2016), Shanghai, 26-29 September 2016.
[13]
Bui, K.H.-T., Duong, T.D., Nguyen, N.T., et al. (2014) Symptomatic Knee Osteoarthritis Treatment Using Autologous Adipose Derived Stem Cells and Platelet-Rich Plasma: A Clinical Study. Biomedical Research and Therapy, 1, 2-8. https://doi.org/10.15419/bmrat.v1i01.11
[14]
Tien Tran Viet, et al. (2015) Research on the Application of Autologous Stem Cells in the Treatment of Osteoarthritis. State-Level Independent Project, Military Medical Academy, Viet Nam.
[15]
Hoai, T.P. (2017) Study on the Results of Treatment of Primary Knee Osteoarthritis with Autologous Adipose Tissue Stem Cell Therapy. Hanoi Medical University, Hà Nội.
[16]
Koh, Y.-G., Jo, S.-B., Kwon, O.-R., et al. (2012) Mesenchymal Stem Cell Injections Improve Symptoms of Knee Osteoarthritis. The Journal of Arthroscopic and Related Surgery, 29, 748-755. https://doi.org/10.1016/j.arthro.2012.11.017
[17]
Jo, C.H., Lee, Y.G., Shin, WH., et al. (2014) Intra-Articular Injection of Mesenchymal Stem Cells for the Treatment of Osteoarthritis of the Knee: A Proof-of-Concept Clinical Trial. Stem Cells, 32, 1254-1266. https://doi.org/10.1002/stem.1634
