This study aims to develop collagen-based hemostatic materials. The sheet-shaped collagen sponge was manufactured by freeze-vacuum drying the aqueous solution of collagen (Col) and heat-denatured collagen (Col’) at a composition ratio of 2/1. The both sides or one side of sponge was treated with ultraviolet (UV) irradiation for 15 minutes to introduce intermolecular crosslinks between collagen molecules. The elution behavior of collagen sponge was investigated by immersing the sponge in water for a predetermined time and then by measuring the weight change. The double-sided UV-irradiated sponge showed very slow elution properties. On the other hand, the single-sided UV-irradiated sponge showed initially fast elution and subsequent very slow elution properties. Such initially fast elution of collagen molecules from the surface without UV-irradiation allows an adhesion of collagen sponge to the wound surface and results in hemostatic effect. In addition, the water absorption and retention properties of sponge were investigated by placing the hydrated sponge on a mesh for a predetermined time and then by measuring the weight change. The double-sided UV-irradiated sponge absorbed 81 times more water than own weight and showed a value of 45 times even after 7 days. The single-sided UV-irradiated sponge absorbed 80 times more water than own weight and showed a value of 39 times even after 7 days. The sponge with high water absorption and retention properties allows a wound healing effect because such sponge can absorb large amounts of blood plasma and exudates containing various cell growth factors. The double-sided UV-irradiated sponge is a good candidate for the wound dressing. On the other hand, the single-sided UV-irradiated sponge is a good candidate for the hemostatic material.
References
[1]
Clark, R.A.F. (1985) Cutaneous Tissue Repair: Basic Biological Considerations. I. Journal of the American Academy of Dermatology, 13, 701-725. https://doi.org/10.1016/S0190-9622(85)70213-7
[2]
Burke, J.F., Yannas, I.V., Quinby, W.C., Bondoc, C.C. and Jung, W.K. (1981) Successful Use of a Physiologically Acceptable Artificial Skin in the Treatment of Extensive Burn Injury. Annals of Surgery, 194, 413-428. https://doi.org/10.1097/00000658-198110000-00005
[3]
Yannas, I.V., Burke, J.F., Orgill, D.P. and Skrabut, E.M. (1982) Wound Tissue Can Utilize a Polymeric Template to Synthesize a Functional Extension of Skin. Science, 215, 174-176. https://doi.org/10.1126/science.7031899
[4]
Suzuki, S., Matsuda, K., Isshiki, N., Tamada, Y., Yoshioka, K. and Ikada, Y. (1990) Clinical Evaluation of a New Bilayer Artificial Skin Composed of Collagen Sponge and Silicone Layer. British Journal of Plastic Surgery, 43, 47-54. https://doi.org/10.1016/0007-1226(90)90044-Z
[5]
Koide, M., Osaki, K., Konishi, J., Oyamada, K., Katakura, T., Takahashi, A. and Yoshizato, K. (1993) A New Type of Biomaterial for Artificial Skin: Dehydrothermally Cross-Linked Composites of Fibrillar and Denatured Collagen. Journal of Biomedical Material Research, 27, 79-87. https://doi.org/10.1002/jbm.820270111
[6]
Kuroyanagi, Y. (2016) Tissue-Engineered Products for Skin Regenerative Medicine. Open Journal of Regenerative Medicine, 5, 61-84. https://doi.org/10.4236/ojrm.2016.53006
[7]
Kuroyanagi, M. and Kuroyanagi, Y. (2017) Tissue-Engineered Products Capable of Enhancing Wound Healing. AIMS Materials Science, 4, 561-581. https://doi.org/10.3934/matersci.2017.3.561
[8]
Coln, D., Horton, J., Ogden, M.E. and Buji, L.M. (1983) Evaluation of Hemostatic Agents in Experimental Splenic Lacerations. American Journal of Surgery, 145, 256-259. https://doi.org/10.1016/0002-9610(83)90074-0
[9]
Postlethwaite, A.E., Seyer, J.M. and Kang, A.H. (1978) Chemotactic Attraction of Human Fibroblasts to Type I, II, and III Collagens and Collagen-Derived Peptides. Proceedings of the National Academy of the Sciences of the United States of America, 78, 871-875. https://doi.org/10.1073/pnas.75.2.871
[10]
Kuroyanagi, Y., Suzuki, R. and Kuroyanagi, M. (2021) Design of Collagen-Based Sponge Device for Use in Oral Surgery. Open Journal of Regenerative Medicine, 10, 31-49. https://doi.org/10.4236/ojrm.2021.103003
[11]
Akane, Yu., Matsuda, Y., Takeda, A., Uchinuma, E. and Kuroyanagi, Y. (2012) Effect of EGF and bFGF on Fibroblast Proliferation and Angiogenic Cytokine Production from Cultured Dermal Substitutes. Journal of Biomaterials Science, 23, 1315-1324. https://doi.org/10.1163/092050611X580463
[12]
Carpenter, G. and Cohen, S. (1979) Epidermal Growth Factor. Annual Review of Biochemistry, 48, 193-216. https://doi.org/10.1146/annurev.bi.48.070179.001205
[13]
John Chen, W.Y. and Abatangelo, G. (1999) Functions of Hyaluronan in Wound Repair. Wound Repair and Regeneration, 7, 79-89. https://doi.org/10.1046/j.1524-475X.1999.00079.x
