Total serum IgD from healthy and sick dogs with leishmaniosis

a Specific L. infantum IFN-γ concentration based on IFN-γ producers and IFN-γ non-producers in healthy non-infected, healthy infected and sick dogs. b IFN-γ concentration after ConA stimulation based on IFN-γ producers and IFN-γ non-producers in healthy non-infected, healthy infected and sick dogs. White circles represent individual data of each dog, boxes represent minimum and maximum values of quartiles, the bands inside represent the second quartile (median). Statistical results of LSA IFN-γ: Group 1 (healthy non-infected) < Group 2 (healthy infected) (Mann-Whitney U-test: Z？=？-8.31, P < 0.0001), Group 1 (healthy non-infected) < Group 3 (sick) (Mann-Whitney U-test: Z？=？-3.81, P？=？0.0001), Group 2 (healthy infected) < Group 3 (sick) (Mann-Whitney U-test: Z？=？-3.28, P？=？0.001). ConA IFN-γ: Group 1(healthy non-infected) < Group 2 (healthy infected) (Mann-Whitney U-test: Z？=？-2.67, P？=？0.008). Abbreviations: LSA, Leishmania soluble antigen; ConA, concanavalin A; IFN-γ, interferon gamm