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- 2019
Oridonin protects LPS-induced acute lung injury by modulating Nrf2-mediated oxidative stress and Nrf2-independent NLRP3 and NF-κB pathwaysDOI: 10.1186/s12964-019-0366-y Keywords: Oridonin, Oxidative stress, Inflammation, Acute lung injury, Akt/Nrf2, MAPK/Nrf2, NOD-like receptor protein 3, NF-κB Abstract: Ori inhibited cytotoxicity and ROS generation in RAW 264.7 cells. a The chemical structure of Oridonin (Ori). b Cells were exposed to Ori (2.5, 5 or 10?μM) for 1?h and then trea ted with or without hydrogen peroxide (300?μM) for another 18?h. Cell viability was determined by MTT assay. c Cells were treated with or without Ori for 18?h, stained with 50?μM of DCFH-DA for 30?min, and subsequently exposed to hydrogen peroxide (300?μM) for 10?min to induce ROS generation. DCF fluorescence intensities were detected by a fluorescence microscope. d and e According to the related instructions, LAL enzyme and LPS-LBP-binding assay were measured by the different absorbance. f and g Cells were exposed to Ori (2.5, 5 or 10?μM) for 1?h, treated with or without LPS (1?μg/ml) for another 18?h and stained with 50?μM of DCFH-DA for 30?min. DCF fluorescence intensities were detected by flow cytometry. All results were expressed as the means ± SEM of three independent experiments. *p?<?0.05 and **p?<?0.01 versus the control group, #p?<?0.05 and ##p?<?0.01 versus LPS grou
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