A competitive CZE method for quality control analysis of multivitamin preparations and veterinary products containing B-group vitamins was developed. Vitamins of interest are thiamine hydrochloride (B1), thiamine monophosphate chloride (B1a), riboflavine (B2), riboflavine-5′monophosphate (B2a), nicotinamide (B3), d-pantothenic acid calcium salt (B5), pyridoxine hydrochloride (B6), folic acid (B9), and 4-aminobenzoic acid (B10). These analytes were separated optimizing the experimental conditions in 20？mM tetraborate buffer as a BGE (background electrolyte), on a Beckman P/ACE System MDQ instrument, using uncoated fused silica capillary. The effective capillary length was of 49.5？cm, ？μm, the applied voltage 20？kV and the temperature 25°C. Detection was performed by a diode array detector at 214？nm for all vitamins except B5 (190？nm) and B2a (260？nm). Separation time was about 9？min. After experimental conditions optimization, the proposed method was validated. Precision of migration time and corrected peak area, linearity range, LOD and LOQ, accuracy (recovery), robustness, and ruggedness were evaluated for each analyte demonstrating the good reliability of the method. Analyses of the pharmaceutical real samples were performed and confirmed the versatility of this method. 1. Introduction Quality control (QC) plays an essential role in the pharmaceutical industry. In fact many analytical methods, employing different techniques, are being developed for evaluation of pharmaceutical preparations. Vitamins of B group were well separated by RP-HPLC. A method for the simultaneous determination of taurine and 10 water-soluble vitamins including vitamin B1 (thiamine), B2 (riboflavine), B5 (pantothenic acid), B6 (pyridoxine and pyridoxal), B8 (biotin), B9 (folic acid), C (ascorbic acid), and B3 (nicotinamide and nicotinic acid) in multivitamin tablets was developed and validated. Detection of components was by ESI-MS [1]. Some water-soluble vitamins of our interest (ascorbic acid, thiamine hydrochloride, riboflavine-5′-phosphate sodium, pyridoxine hydrochloride, nicotinamide, and (+)-panthenol) and two preservatives (methylparaben and sodium benzoate) in multivitamin syrup were well-separated on Zorbax SB-Aq (C18) column [2]. A HPLC-UV method was established for the simultaneous determination of eight vitamins, including B1, B2, B3, B6, B9, cyanocobalamin, ascorbic acid in baby milk powder [3]. A validated HPLC-UV method for the determination of seven B-complex vitamins (B1, B2, B3, B6, B9, and cyanocobalamin) in pharmaceuticals and biological fluids after
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