马尾松花粉提取物对前列腺肥大小鼠的预防作用及代谢组学研究

探究马尾松花粉提取物(MPPE)对前列腺肥大(BPH)小鼠的影响。雄性昆明种小鼠随机分为6组，分别为空白对照组、BPH组、20、40和80 mg/kg?d MPPE组及非那雄胺(0.8 mg/kg?d)组。预先灌胃MPPE两周之后，在腹腔注射丙酸睾酮建模的同时连续灌胃MPPE或非那雄胺四周。实验结束后观察组织形态变化，测定细胞因子IL-1β、IL-6、TNF-α、PACP、DHT、NO及NOS的水平。利用UPLC-QTOF-MS技术检测小鼠血清、尿液中代谢物的变化，采用PLS-DA分类，寻找潜在生物标志物。三种剂量的MPPE处理均降低了前列腺指数、炎性细胞因子、DHT浓度及PACP、NOS的活性，并减少了NO的生成。血清代谢组学鉴定了3个潜在生物标记物，分别为1-棕榈酰溶血磷脂酰胆碱、1-O-十六烷基-2-O-乙酰基-sn-甘油基-3-磷酸胆碱和(Z)-13-二十二烯酰胺。马尾松花粉提取物可以明显降低BPH病理症状及相关生化指标，调节标志性代谢物的水平。其可能的作用机制与抑制炎症及缓解脂质代谢紊乱相关。
The effect of masson pine (Pinus massoniana) pollen extract (MPPE) on benign prostate hypertrophy (BPH) in mice was studied. Male KM mice were randomly divided into six groups: normal control group, BPH model group, 20, 4080 mg/kg MPPE groups and 0.8 mg/(kg?d) finasteride group. After two weeks of advanced intragastric MPPE administration, an intraperitoneal injection of testosterone propionate was used to initiate the BPH, following which the MPPE or finasteride treatment was administered intragastically for 4 weeks. At the end of the treatment period, morphological changes in the tissues were observed, and levels of the cytokines IL-6, IL-1β, and TNF-α as well as of PACP, DHT, NO, and NOS were measured. Ultraperformance liquid chromatography-mass spectrometry was employed to detect changes in serum and urine metabolites. Partial least squares discriminant analysis was used for group differentiation and biomarker selection. All three doses of MPPE decreased the prostate index, inflammatory cytokines, DHT concentration, PACP and NOS activities, and NO production. Serum metabolomics analysis found and identified three potential biomarkers: 1-palmitoyl-sn-glycero-3-phosphocholine, 1-O-hexadecyl-2-O-acetyl-sn-glyceryl-3-phosphorylcholine, and cis-13-docosenoamide. MPPE could significantly reduce the pathological symptoms and biochemical parameters of BPH and adjust the level of the marker metabolites. The mechanism may be related to the inhibition of inflammation and alleviation of lipid metabolism disorders.