Studies regarding the influence of certain inorganic pollutants on the quality of the soil from mining areas in Suceava county

The correct functioning of the soil is assured by the biologic activity of the microorganisms at this level and the enzymatic activity reflects the metabolic processes that take place in various environmental conditions, being also a very good indicator for both natural and anthropic disturbances, presenting a quick response to the changes induced by these in the soil ecosystem. The precise identification of the effects caused by sulphur exploitation and its contamination with heavy metals on certain microbiological parameters (the activity of the isocitrate-dehydrogenase, - ketoglutarate-dehydrogenase, succinate-dehydrogenase, malate-dehydrogenase) is the main purpose of this study. The experiment was made on probes sampled from the Climani Mountains, Tarnia and Rdui (the last is considered to be unpolluted area), both in-depth as well as on the soil surface. The method that was used is based mainly on the capacity of the dehydrogenases to transfer the excess hydrogen from different substrates to 2,3,5-triphenil-tetrazolium chloride, that can be reduced and goes into red colored triphenil-formazane, the intensity of the formazane`s color being determined spectrophotometrically. The analysis of the obtained results show that the level of activity of the microorganisms in the soil, expressed trough the global dehydrogenasic activity of the microbial consortium, can be considered as an indicator to the situation of the examined ecosystem, reflected by the disponibility of the organic matter on soil surface and by the intensity of the transformation and circulation process of the organic matter.