Proton spectrum analysis of 4-(ethylamino)-6-methyl-7-propylpyrido[3,4- d]pyrimidin-8(7H)-one using 1-D NOE technique and Correlation spectroscopy methodology.

The power of Nuclear Magnetic Resonance spectroscopy (NMR) in structure elucidation derives in large part from its ability to establish bonding connectivity (via J- coupling interaction) or through space proximity (via dipolar coupling interactions) of nuclei the first is known as Correlation spectroscopy(COSY) and the second being Nuclear Overhauser effect spectroscopy(NOESY). The amount of time consumed in elucidating a structure depends on the rate at which these interaction can be detected by NMR and analyzed.1D NMR methods most often explore interactions between only few nuclei at a time: spin-spin decoupling measurements are used to demonstrate through-bond connectivity; and NOE measurements are used to probe inter-nuclear distances, 2-Dimensional NMR experiments provide much more structural information in a given time period.