Study of nanofluids is important for different types of heat transfer management systems. Cupric oxide nanoparticles (CuO NPs) were prepared by the chemical route and different nanofluid samples of CuO NPs dispersed in PVA in dif- ferent concentrations were prepared using ultrasonication. The apparatus acoustic particle sizer (APS-100) was used to make high precision measurements of the ultrasonic attenuation depending upon different frequencies in the frequency range 48 to 99 MHz. The ultrasonic attenuation data are inverted to particle size distribution (PSD) and are used for particle size determination of CuO NPs. Temperature dependent ultrasonic velocity in the samples is also measured. The results of ultrasonic spectroscopy are compared with the microscopic measurements such as transmission electron microscopy (TEM) and X-ray diffraction (XRD). There is good agreement between data produced by ultrasonic spec- troscopy and the microscopic measurements.
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