All Title Author
Keywords Abstract

Publish in OALib Journal
ISSN: 2333-9721
APC: Only $99

PREPRINT - OALib PrePrints is not a peer-reviewed venue.

Biosynthesis of Copper Nanoparticles and Their Antimicrobial Activity

DOI: 10.4236/oalib.preprints.1200067, PP. 1-15

Subject Areas: Environmental Chemistry, Green Chemistry, Biological Chemistry, Analytical Chemistry, Nanometer Materials

Keywords: Copper Nanoparticle, CuNps, Antimicrobial, Green Synthesis

Full-Text   Cite this paper   Add to My Lib


Development of green nanotechnology is generating interest of researchers toward ecofriendly biosynthesis of nanoparticles. Biomolecules present in plant extracts can be used to reduce metal ions to nanoparticles in a single-step green synthesis process. This biogenic reduction of metal ion to base metal is quite rapid, readily conducted at room temperature and pressure, and easily scaled up. In this study, biosynthesis of stable copper nanoparticles were done using datura meta leaf extract. These biosynthesized Cu nanoparticles  were characterized by UV/Vis-spectroscopy, Particle size analyzer (PSA), Transmission electron mictroscopy (TEM), Energy dispersive X-ray Analysis (EDX),Fourier transform infrared spectroscopy (FTIR). It was observed that the datura meta leaf extract can reduce copper ions into copper nanoparticles within 8 to 10 min of reaction time. Thus, this method can be used for rapid and ecofriendly biosynthesis of stable copper nanoparticles.Synthesis mediated by plant extracts is environmentally benign. The reducing agents involved include the various water soluble plant metabolites (e.g. alkaloids, phenolic compounds, terpenoids) and co-enzymes. Extracts of a diverse range of plant species have been successfully used in making nanoparticles. In addition to plant extracts, live plants can be used for the synthesis. It was found that copper nanoparticles were also found to exhibit reasonably good antimicrobial activity when compared with standard Chloramphenicol, which suggests its potential use as antimicrobial agent. Hence, there is scope to develop new methods for the synthesis of nanoparticles which should be required inexpensive reagent, less drastic reaction condition and eco-friendly. 

Cite this paper

Parikh, P. , Zala, D. and Makwana, B. A. (2014). Biosynthesis of Copper Nanoparticles and Their Antimicrobial Activity. Open Access Library PrePrints, 1, e067. doi:


[1]  J. M. Taylor, "New dimensions for manufacturing: a UK strategy for nanotechnology," Ed., DTIC Document, 2002.
[2]  A Helland, "Nanoparticles: a closer look at the risks to human health and the environment," Perceptions and precautionary measures of industry and regulatory bodies in Europe, 2004.
[3]  G. B. Sergeev, Nanochemistry, Elsevier Science, 2006.
[4]  A. P. Alivisatos, "Perspectives on the physical chemistry of semiconductor nanocrystals," The Journal of Physical Chemistry, vol. 100, no. 31, pp. 13226-13239, 1996.
[5]  G. B. Sergeev and T. I. Shabatina, "Cryochemistry of nanometals," Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 313, pp. 18-22, 2008.
[6]  R. Jin, Y. W. Cao, C. A. Mirkin, K. Kelly, G. C. Schatz and J. Zheng, "Photoinduced conversion of silver nanospheres to nanoprisms," Science, vol. 294, no. 5548, pp. 1901, 2001.
[7]  L. A. Peyser, A. E. Vinson, A. P. Bartko and R. M. Dickson, "Photoactivated fluorescence from individual silver nanoclusters," Science, vol. 291, no. 5501, pp. 103-106, 2001.
[8]  S. Sun, C. Murray, D. Weller, L. Folks and A. Moser, "Monodisperse FePt nanoparticles and ferromagnetic FePt nanocrystal superlattices," Science, vol. 287, no. 5460, pp. 1989-1992, 2000.
[9]  E. Mayes, A. Bewick, D. Gleeson, J. Hoinville, R. Jones, O. Kasyutich, A. Nartowski, B. Warne, J. Wiggins and K. Wong, "Biologically derived nanomagnets in self-organized patterned media," Magnetics, IEEE Transactions on, vol. 39, no. 2, pp. 624-627, 2003.
[10]  M. Han, X. Gao, J. Z. Su and S. Nie, "Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules," Nature biotechnology, vol. 19, no. 7, pp. 631-635, 2001.
[11]  J. Wang, "Nanoparticle-based electrochemical DNA detection," Analytica Chimica Acta, vol. 500, no. 1-2, pp. 247-257, 2003.
[12]  M. Moreno-Manas and R. Pleixats, "Formation of carbon-carbon bonds under catalysis by transition-metal nanoparticles," Accounts of chemical research, vol. 36, no. 8, pp. 638-643, 2003.
[13]  T. Yamada, Y. Iwasaki, H. Tada, H. Iwabuki, M. Chuah, T. VandenDriessche, H. Fukuda, A. Kondo, M. Ueda and M. Seno, "Nanoparticles for the delivery of genes and drugs to human hepatocytes," Nature biotechnology, vol. 21, no. 8, pp. 885-890, 2003.
[14]  R. A. Freitas, "What is nanomedicine?," Nanomedicine: Nanotechnology, Biology and Medicine, vol. 1, no. 1, pp. 2-9, 2005.
[15]  J. A. Rojas-Chapana and M. Giersig, "Multi-walled carbon nanotubes and metallic nanoparticles and their application in biomedicine," Journal of nanoscience and nanotechnology, vol. 6, no. 2, pp. 316-321, 2006.
[16]  E. C. Wang and A. Z. Wang, "Nanoparticles and their applications in cell and molecular biology," Integrative Biology, vol. 6, no. 1, pp. 9-26, 2014.
[17]  N. Li, P. Zhao and D. Astruc, "Anisotropic Gold Nanoparticles: Synthesis, Properties, Applications, and Toxicity," Angewandte Chemie International Edition, 2014.
[18]  R. Narayanan and M. A. El-Sayed, "Effect of nanocatalysis in colloidal solution on the tetrahedral and cubic nanoparticle shape: electron-transfer reaction catalyzed by platinum nanoparticles," The Journal of Physical Chemistry B, vol. 108, no. 18, pp. 5726-5733, 2004.
[19]  Subhankari and P. Nayak, "Synthesis of Copper Nanoparticles Using Syzygium aromaticum (Cloves) Aqueous Extract by Using Green Chemistry," World, vol. 2, no. 1, pp. 14-17, 2013.
[20]  T. Wang, X. Long, Y. Cheng, Z. Liu and S. Yan, "The potential toxicity of copper nanoparticles and copper sulphate on juvenile< i> Epinephelus coioides," Aquatic Toxicology, vol. 152, pp. 96-104, 2014.
[21]  H. Choi and S.-H. Park, "Seedless growth of free-standing copper nanowires by chemical vapor deposition," Journal of the American Chemical Society, vol. 126, no. 20, pp. 6248-6249, 2004.
[22]  Fang, H. Niu, T. Lin and X. Wang, "Applications of electrospun nanofibers," Chinese Science Bulletin, vol. 53, no. 15, pp. 2265-2286, 2008.
[23]  N. A. Dhas, C. P. Raj and A. Gedanken, "Synthesis, characterization, and properties of metallic copper nanoparticles," Chemistry of Materials, vol. 10, no. 5, pp. 1446-1452, 1998.
[24]  N. V. Surmawar, S. R. Thakare and N. Khaty, "One-Pot, Single Step Green Synthesis of Copper Nanoparticles: SPR Nanoparticles," International Journal of Green Nanotechnology, vol. 3, no. 4, pp. 302-308, 2011.
[25]  S. Gunalan, R. Sivaraj and R. Venckatesh, "< i> Aloe barbadensis Miller mediated green synthesis of mono-disperse copper oxide nanoparticles: Optical properties," Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, vol. 97, pp. 1140-1144, 2012.
[26]  S. Pal, Y. K. Tak and J. M. Song, "Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gram-negative bacterium Escherichia coli," Applied and environmental microbiology, vol. 73, no. 6, pp. 1712-1720, 2007.
[27]  E. K. Perry, "Plants of the gods," Neurochemistry of consciousness: Neurotransmitters in mind. Advances in Consciousness Research, vol. 36, pp. 205-225, 2002.
[28]  Edley, "Brugmansia x candida–White Angel’s Trumpet."
[29]  B. Siklos, "Datura rituals in the Vajramahabhairava-Tantra," Curare, vol. 16, no. 2, pp. 71-76, 1993.
[30]  J. Edley, "Datura metel–Indian Thorn Apple."
[31]  S. Link and M. A. El-Sayed, "Size and temperature dependence of the plasmon absorption of colloidal gold nanoparticles," The Journal of Physical Chemistry B, vol. 103, no. 21, pp. 4212-4217, 1999.
[32]  Y. H. Kim, Y. S. Kang, W. J. Lee, B. G. Jo and J. H. Jeong, "Synthesis of Cu nanoparticles prepared by using thermal decomposition of Cu-oleate complex," Molecular Crystals and Liquid Crystals, vol. 445, no. 1, pp. 231/[521]-238/[528], 2006.
[33]  J. S. Kim, E. Kuk, K. N. Yu, J. H. Kim, S. J. Park, H. J. Lee, S. H. Kim, Y. K. Park, Y. H. Park and C. Y. Hwang, "Antimicrobial effects of silver nanoparticles," Nanomedicine: Nanotechnology, Biology and Medicine, vol. 3, no. 1, pp. 95-101, 2007.
[34]  Q. Feng, J. Wu, G. Chen, F. Cui, T. Kim and J. Kim, "A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus," Journal of biomedical materials research, vol. 52, no. 4, pp. 662-668, 2000.
[35]  F. Raimondi, G. G. Scherer, R. Kotz and A. Wokaun, "Nanoparticles in energy technology: Examples from electrochemistry and catalysis," Angewandte Chemie International Edition, vol. 44, no. 15, pp. 2190-2209, 2005.


comments powered by Disqus

Contact Us


微信:OALib Journal