Simultaneous determination of non-steroidal anti-inflammatory drugs in pharmaceutical formulations and human serum by reversed phase high performance liquid chromatography

a rapid and sensitive method using high performance liquid chromatography has been developed and validated for the simultaneous determination of non-steroidal anti-inflammatory drugs (nsaids) in pharmaceutical formulations and human serum. six nsaids including: naproxen sodium, diclofenac sodium, meloxicam, flurbiprofen, tiaprofenic and mefenamic acid were analyzed simultaneously in presence of ibuprofen as internal standard on mediterranea c18 (5 μm, 250 x 0.46 mm) column. mobile phase comprised of methanol: acetonitrile: h2o (60:20:20, v/v; ph 3.35) and pumped at a flow rate of 1 ml min-1 using 265 nm uv detection. the method was linear over a concentration range of 0.25-50 μg ml-1 (r2 = 0.9999).