Plant regeneration of Mullein Nightshade (Solanum donianum Walp.) from leaf explants

a reliable protocol for plant regeneration of mullein nightshade (solanum donianum walp.) was developed from in vitro leaf explants. they were cultured on murashige and skoog semisolid medium, supplemented with several combinations of zeatin riboside and 1-naphthaleneacetic acid for shoot regeneration. the formulation that originated most shoots per explant was 8.5 μm zeatin riboside in absence of 1-naphthalene acetic acid. explants with shoots were transferred to murashige and skoog medium, with half the normal salt concentration and without plant growth regulators for elongation. elongated shoots were individualized, and they rooted readily in half-strength murashige and skoog medium without plant growth regulators. the regenerated plants, which were transferred to soil in a greenhouse, followed a similar phenological pattern to plants grown from seed.