Método de cultivo de larvas de moscas Lucilia sericata para terapia larval

the general objectives of the present study were; 1) describe the developmental cycle of lucilia sericata (calliphoridae) though the egg, larva, pupa, and imago stages, and 2) develop a method for disinfecting the eggs of l. sericata. the developmental process of this fly was observed daily, determining times required for the appearance of each different stage. the disinfection technique included successive treatment of the eggs in solutions of 0.5% sodium hypochlorite and 10% formalin after the eggs were obtained from experimentally crossing male and female flies in the laboratory. the eggs were then rinsed in sterile 0.15 mnacl, and egg samples were spread-plated on trypticase soya agar containing 5% sheep blood, and also inoculated into in nutrient broth.the eggs of l. sericata hatched after 15 ± 3.5 hr, producing pupae after 138 ± 17 hr, and the adults emerged after 126 ± 21 hr. the disinfection technique used in the present study was 87% effective. it was concluded that that the complete development as well as the disinfection of the eggs could be carried out successfully in the laboratory for the production of larvae useful in maggot therapy