Determining plasma morphine levels using GC-MS after solid phase extraction to monitor drug levels in the postoperative period

objective: to implement a selective and sensitive analytical method to quantify morphine in small volumes of plasma by gas-liquid chromatography-mass spectrometry (gc-ms), aimed at post-operatively monitoring the drug. method: a gas-liquid chromatographic method with mass detection has been developed to determine morphine concentration in plasma after solid phase extraction. morphine-d3 was used as an internal standard. only 0.5 ml of plasma is required for the drug solid-phase extraction in the bond elut-certify？, followed by the quantification of morphine derivative by gc-ms using a linear temperature program, a capillary fused silica column, and helium as the carrier and make-up gas. the method was applied to determine morphine content in plasma samples of four patients during the postoperative period of cardiac surgery. patient-controlled analgesia with morphine was performed by a venous catheter, and a series of venous blood samples were collected. after the oro-after the orotracheal extubation, morphine plasma levels were monitored for up to 36 hours. results: the run time was 16 minutes because morphine and the internal standard were eluted after 8.8 minutes. the gc-ms method had 0.5 -1000 ng/ml linearity range (r2=0.9995), 0.1 ng/ml limit of detection, intraday and interday precision equivalent to 1.9% and 6.8%, and 0.1% and 0.8% systematic error (intraday and interday, respectively). the analytical method showed optimal absolute (98%) and relative (100.7%) recoveries. morphine dose requirements and plasma levels are discussed. conclusion: the analytical gas-liquid chromatography-mass spectrometry method is selective and adequate for morphine measurements in plasma for applications in clinical studies.