Identificación de cultivares de Medicago sativa L. por caracteres bioquímicos en semillas y plántulas

since alfalfa cultivar seeds are morphologically similar, seed and seedling protein analysis would be adequate to differentiate both cultivars. the purpose of this study was to characterize alfalfa cultivars from two different harvests according to seed protein level determined through sds page and a page, and in protrusion and cotyledonar state through dehidrogenase alcohol isoenzyme. the adequate sample size for electrophoresis was 0.5g of seeds in 5.00ml of extraction buffer and the appropriate sowing volume was 10μl. in both sds page and a page methods, protein fractioning was carried out though it was not possible to differentiate among cultivars; the slight differences found were not repeated in samples from different harvest years. the adh system showed differences among cultivars getting a higher differentiation at the protrusion state.