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Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA

DOI: 10.1590/S1517-83822012000100047

Keywords: hpv, cervical cancer, fta elute, molecular detection.

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Abstract:

this study aimed to evaluate the use of the fta elute cardtm impregnated with cervicovaginal sample directly in the pcr amplification for detection of hpv-dna. the results were compared to a reference technique. this method was more efficient than the protocol indicated by the manufacturer, identifying 91.7% against 54.2% of the positive samples.

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