Bioremediation of herbicide velpar K？ in vitro in aqueous solution with application of EM-4 (effective microorganisms)

this work assessed the bioremediation of herbicide velpar k？, in vitro in aqueous solution, used against weeds in sugar cane in s？o paulo state. the herbicide contained hexazinone and diuron. it was used the microbial inoculant denominated effective microorganisms (em-4), pool of microorganisms from soil that contained lactic and photosynthetic bacteria, fungi, yeasts and actinomycetes for bioremediation. results for the depth of cultivation on agar-agar inoculated with em-4 showed the microorganisms growth in the concentrations between 0.2% and 1.0% of the velpar k？in the gel. the analysis of high performance liquid chromatography (hplc) showed that the em-4 was effective for the bioremediation of the herbicide, which reached the values of 80% for diuron and 70% for hexazinone after 21 days in solution of 2:1 of velpar k？/em-4 ratio. these results could be useful for planning the bioremediation of contaminated areas with velpar k？.