Actividad Antioxidante de Hidrolizados Enzimáticos de Plasma Bovino Obtenidos por Efecto de Alcalasa？ 2.4 L

the antioxidant activity of bovine plasma hydrolysates (bph) obtained with alcalase 2.4 l at different hydrolysis degrees was evaluated. the hydrolysates were fractioned through ultrafiltration membranes and were purified by ion-exchange chromatography, with subsequent reverse-phase high performance liquid chromatography. the results showed that the hydrolysates of bovine plasma obtained under the established hydrolysis conditions, had strong scavenging ability on 2,2-azino bis-(3-ethylbenzothiazoline)-6-sulfonic acid free radicals and a great reduction power compared with proteins of non-hydrolyzed plasma. additionally, it was found that the antioxidant activity increases as function of the hydrolysis degree, and that this activity remains after in-vitro digestion. the processes described allow obtaining a peptid with antioxidant activities similar to that of conventional commercial antioxidants.