Use of seed protein polymorphism for discrimination of improvement level and geographic origin of upland rice cultivars

grain proteins from 58 brazilian and nine japanese upland rice cultivars (oryza sativa l.) were electrophoretically separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). densitometric scanning of the electrophoretic profiles permitted the estimation of the relative concentration of 16 protein fractions, which were used as variables for the calculation of fisher's canonical discriminating functions. significant differences between mean values of protein fractions were useful in distinguishing brazilian and japanese cultivars, as well as improved and non-improved brazilian rice cultivars in scattered plots. electrophoretically detectable protein polymorphism in rice grain can indicate geographic origin as well as breeding improvement level of a cultivar. improved cultivars were those released by plant breeding institutes.