High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica

high performance enzymatic synthesis of oleyl oleate, a liquid wax ester was carried out by lipase-catalysed esterification of oleic acid and oleyl alcohol. various reaction parameters were optimised to obtain high yield of oleyl oleate. the optimum condition to produce oleyl oleate was reaction time; 5 min, organic solvents of log p ≥ 3.5, temperature; 40-50oc, amount of enzyme; 0.2-0.4 g and molar ratio of oleyl alcohol to oleic acid; 2:1. the operational stability of enzyme was maintained at >90% yield up to 9 cycles. analysis of the yield of the product showed that at optimum conditions, >95% liquid wax esters were produced