Detec？？o do Sugarcane mosaic virus no Paraná e limpeza somaclonal por cultura de tecidos

an isolate of sugarcane mosaic virus (scmv) associated with mosaic symptoms in the rb925268 sugarcane clone was identified in paraná state. axilary buds of 4 to 8 mm used as explants were regenerated into plantlets using ms medium with and without ribavirin antiviral, at concentrations of 10 to 60 mg/l. ribavirin was toxic to sugarcane at all concentrations, resulting in plant death at concentrations of 30 mg/l or higher. the complete elimination of scmv was obtained in ms medium with 25 mg/l ribavirin concentration, confirmed by sap inoculation into sorghum bicolor cv. rio and by rt-pcr.