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Biosalud  2010 

COMPORTAMIENTO DE LOS NIVELES DE IgM e IgG EN CERDAS DE CRíA EN VARIAS PORCíCOLAS DEL EJE CAFETERO COLOMBIANO VACUNADAS CONTRA LEPTOSPIRA CON UNA VACUNA PENTAVALENTE

Keywords: leptospira, immunological techniques, swine, indirect fluorescent antibody technique, agglutination test, immunoglobulin m, immunoglobulin g.

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Abstract:

the goal of this study was to determine the levels of postvaccinal antibodies against several serovars of leptospira in 78 sows in two different gestation moments (day 90 to 95: moment 1, and day 110 to 114: moment 2), using the micro agglutination (mat) and indirect immunofluorescence (ifi) techniques. each serum was tested for six serovars of leptospira (canicola, hardjo bovis, pomona, grippotyphosa, icterohaemorrhagiae and bratislava) donated by the center of diagnostics of the instituto colombiano agropecuario (ica) in manizales-caldas-colombia. the results showed a 59% positive sera using mat in moment one and a 17% in moment two, with icterohaemorrhagiae and hardjo bovis as the most prevalent. by means of the ifi technique 100% of sera were positive in both moments, for at least one of the serovars tested, with titers between 1:64 and 1:512 in the first moment and a strong decrease or absence of the titers in the second moment; the most prevalent serovars for ifi were icterohaemorrhagiae and canicola. in conclusion, this vaccine has a low protection capacity since an increase in the number of seropositive sows with igm was observed against some serovars of leptospira in the second moment, as well as an increase in the levels of igg against icterohaemorrhagiae in the second moment, and its low protection capacity in offspring in their first days of life associated with a decrease of the antibodies at the end of the gestation.

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