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Biossensor amperométrico para determina??o de peróxido de hidrogênio em leite

DOI: 10.1590/S0100-46702011000200008

Keywords: peroxidase radish, screen printed, glutaraldehyde, biosensor.

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Abstract:

an amperometric biosensor was developed for detection of hydrogen peroxide in milk samples. the biosensor was constructed from the immobilization of peroxidase on printed carbon electrode. optimization parameters were evaluated in order to obtain better performance of the biosensor. the biosensor showed linearity in the range 5.0 to 40.0 μ mol l-1h2o2 in phosphate buffer. milk samples without dilution reported the detection limit for h2o2 of 0.42 μmol l-1 and quantification of 1.39 μmol l-1.the biosensor could be a sensitive and inexpensive alternative for the detection of h2o2 in adulterated milk samples.

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