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Caracterización molecular de 15 aislamientos de Beauveria bassiana asociados con Cosmopolites y Metamasius en plátano y banano en tres regiones de Colombia

Keywords: entomopathogen, deuteromicetes, molecular markers, rams, genetic diversity, weevils, coleoptera rhinchophorinae, musa spp.

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Abstract:

weevils cosmopolites and metamasius in municipalities of the valle del cauca, caldas and quindío departaments of colombia were collected. monosporic cultures were obtained from10-10 and 10-11 dilutions of beauveria bassiana. isolates were kept at -80°c with 10% glycerol and dna stored at -20°c. rams molecular markers generated a total of 82 fragments of which 67% were polymorphic. a heterozygosity value of 0.24 indicated a medium - high diversity. five groups were formed which have a similarity value of 0.84 and one big group with 11 isolates and four groups with only one isolate. in the big group was detected a duplicate and fungi genetic diversity in the sampled places. neither relationship among isolates of cosmopolites and metamasius nor geographical zone related to the formation of genetic groups.

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