Establecimiento de un protocolo de permeabilización con detergentes que incrementa la eficiencia en la producción de betacianinas en cultivos de células de Beta vulgaris L

red beet (beta vulgaris l.) cell suspensions were permeabilized by means of four chemical detergent agents, tween 20, 40 and 80, and triton x-100？, to evaluate the recovery of betacianins (bc). the permeabilizating agent was selected as a function of the quantity of bc released and the contact time. betacianin concentration was measured using digital color image analysis. the results showed that 36% of betacianins was released using triton x-100？ (0.7mm) during 10min; using these extraction conditions, the viability remained at 60-70%. this treatment allowed a second growing-cycle, as well as, an additional accumulation of betacianins.