Vector control methods have significantly reduced malaria transmission. However, challenges such as insecticide resistance and behavioral adaptation of vectors highlight the urgent need for alternative control measures. Novaluron, an insect growth regulator, was evaluated following the World Health Organization Pesticide Evaluation Scheme. Laboratory bioassays were carried out using third (L3) fourth instar (L4) larvae and pupae of Anopheles coluzzii and Anopheles arabiensis. At the experimental station, efficacy and residual activity were assessed exclusively on L3, with multiple diagnostic doses (DD) for each species. In the laboratory, emergence inhibition (EI) 50% and 99% values on L3 were 0.145 and 0.29 μg/l for An. coluzzii and 0.37 and 0.69 μg/l for An. Arabiensis, on L4, 1.95 and 3.61 μg/l for An. coluzzii and 2.76 and 5.02 μg/l for An. arabiensis. For pupae, EI50 and EI99 values were 145 and 265 μg/l for An. coluzzii and 428.37 and 714.13 μg/l for An. arabiensis. At the experimental station, An. coluzzii showed respectively 86% and 77,08% cumulative mortality (CM) and emergence inhibition rate (EIR) by day 33 at 6 μg/l. For An. arabiensis, 84% CM and 77.08% EIR were observed by day 40 at 15 μg/l. Anopheles coluzzii and An. arabiensis are susceptible to Novaluron at low concentrations under laboratory conditions. At optimal doses, Novaluron effectively inhibits both pupation and adult emergence.
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