Background: To avoid the detrimental effects of solar skin exposure, including hyperpigmentation, skin laxity and loss of radiance, dermatologists routinely recommend sun avoidance and the daily use of sunscreens. Repair of sun damaged skin is usually addressed in terms of ablative or surgical procedures. Compared to the well documented effects of ultraviolet radiation, oxidative stress related to environmental conditions, including solar exposure, diet and lifestyle is often overlooked as a cause of skin damage and premature aging. Purpose: This paper aims to investigate the efficacy of a topical formulation comprised of a comprehensive antioxidant complex, an environmental protection complex and an optimized delivery system to enhance overall skin physiological and cellular antioxidant and anti-inflammatory protection from solar and environmental damage. Methods: The Formulation (#2LCRE.20) was tested for: in-vitro LDH cytotoxicity activity, qPCR gene expression on a 3-dimensional skin model, in-vivo efficacy on 21 patients introducing the Formulation into their pre-established skincare regimen with no other treatments and in-vivo experience testing on 7 subjects incorporating the Formulation into their pre-established skincare ritual including a weekly hyperpigmentation facial treatment in a professional skin clinic. Results:In-vivo data showed a significant improvement in skin hydration, hyperpigmentation, radiance, inflammation and redness after exposure to the Formulation. These results were supported by in-vitro testing, where six specific genes were upregulated. Conclusion: This study highlights the role that oxidation and antioxidant skin Formulations and Treatments can play in helping to prevent the damaging effects of solar and environmentally induced skin damage.
References
[1]
Tanaka, Y. (2019) Long-Term Objective Assessments of Skin Rejuvenation Using Solar Protection and Solar Repair Shown through Digital Facial Surface Analysis and Three-Dimensional Volumetric Assessment. Clinical, Cosmetic and Investigational Dermatology, 12, 553-561. https://doi.org/10.2147/ccid.s218176
[2]
Tanaka, Y. (2020) Three-Dimensional Quantification of Skin Surface Displacement Following Skin Rejuvenation Using Solar Protection and Solar Repair. Journal of Clinical and Aesthetic Dermatology, 13, 47-50.
[3]
Tanaka, Y. (2012) Impact of Near-Infrared Radiation in Dermatology. World Journal of Dermatology, 1, 30-37. https://doi.org/10.5314/wjd.v1.i3.30
[4]
Tanaka, Y. and Gale, L. (2013) Beneficial Applications and Deleterious Effects of Near-Infrared from Biological and Medical Perspectives. Optics and Photonics Journal, 3, 31-39. https://doi.org/10.4236/opj.2013.34a006
[5]
Tanaka, Y. and Gale, L. (2015) Protection from Near-Infrared to Prevent Skin Damage. Optics and Photonics Journal, 5, 113-118. https://doi.org/10.4236/opj.2015.54010
[6]
Tanaka, Y. (2023) Photoprotective Ability of Sunscreens against Ultraviolet, Visible Light and Near-Infrared Radiation. Optics and Photonics Journal, 13, 140-146. https://doi.org/10.4236/opj.2023.136012
[7]
Tanaka, Y. and Nakayama, J. (2016) Upregulated Epidermal Growth Factor Receptor Expression Following Near-Infrared Irradiation Simulating Solar Radiation in a Three-Dimensional Reconstructed Human Corneal Epithelial Tissue Culture Model. Clinical Interventions in Aging, 11, 1027-1033. https://doi.org/10.2147/cia.s111530
[8]
Jin, S., Li, Z., Choi, E.K., Lee, S., Kim, Y.K., Seo, E.Y., et al. (2018) Urban Particulate Matter in Air Pollution Penetrates into the Barrier-Disrupted Skin and Produces Ros-Dependent Cutaneous Inflammatory Response in Vivo. Journal of Dermatological Science, 91, 175-183. https://doi.org/10.1016/j.jdermsci.2018.04.015
[9]
Paik, K., Na, J., Huh, C. and Shin, J. (2024) Particulate Matter and Its Molecular Effects on Skin: Implications for Various Skin Diseases. International Journal of Molecular Sciences, 25, Article 9888. https://doi.org/10.3390/ijms25189888
[10]
Dijkhoff, I.M., Drasler, B., Karakocak, B.B., Petri-Fink, A., Valacchi, G., Eeman, M., et al. (2020) Impact of Airborne Particulate Matter on Skin: A Systematic Review from Epidemiology to in Vitro Studies. Particle and Fibre Toxicology, 17, Article No. 35. https://doi.org/10.1186/s12989-020-00366-y
[11]
Juan, C.A., Pérez de la Lastra, J.M., Plou, F.J. and Pérez-Lebe？a, E. (2021) The Chemistry of Reactive Oxygen Species (ROS) Revisited: Outlining Their Role in Biological Macromolecules (DNA, Lipids and Proteins) and Induced Pathologies. International Journal of Molecular Sciences, 22, Article 4642. https://doi.org/10.3390/ijms22094642
[12]
Silva, S.A.M.E., Michniak-Kohn, B. and Leonardi, G.R. (2017) An Overview about Oxidation in Clinical Practice of Skin Aging. Anais Brasileiros de Dermatologia, 92, 367-374. https://doi.org/10.1590/abd1806-4841.20175481
[13]
Bazin, R. and Fanchon, C. (2006) Equivalence of Face and Volar Forearm for the Testing of Moisturizing and Firming Effect of Cosmetics in Hydration and Biomechanical Studies. International Journal of Cosmetic Science, 28, 453-461. https://doi.org/10.1111/j.1467-2494.2006.00352.x
[14]
Velez-DelValle, C., Marsch-Moreno, M., Castro-Mu？ozledo, F. and Kuri-Harcuch, W. (2011) Decorin Gene Expression and Its Regulation in Human Keratinocytes. Biochemical and Biophysical Research Communications, 411, 168-174. https://doi.org/10.1016/j.bbrc.2011.06.122
[15]
Kirschner, N., Bohner, C., Rachow, S. and Brandner, J.M. (2010) Tight Junctions: Is There a Role in Dermatology? Archives of Dermatological Research, 302, 483-493. https://doi.org/10.1007/s00403-010-1058-z
[16]
Kirschner, N., Poetzl, C., von den Driesch, P., Wladykowski, E., Moll, I., Behne, M.J., et al. (2009) Alteration of Tight Junction Proteins Is an Early Event in Psoriasis: Putative Involvement of Proinflammatory Cytokines. The American Journal of Pathology, 175, 1095-1106. https://doi.org/10.2353/ajpath.2009.080973
[17]
Volksdorf, T., Heilmann, J., Eming, S.A., Schawjinski, K., Zorn-Kruppa, M., Ueck, C., et al. (2017) Tight Junction Proteins Claudin-1 and Occludin Are Important for Cutaneous Wound Healing. The American Journal of Pathology, 187, 1301-1312. https://doi.org/10.1016/j.ajpath.2017.02.006
[18]
Safferling, K., Sütterlin, T., Westphal, K., Ernst, C., Breuhahn, K., James, M., et al. (2013) Wound Healing Revised: A Novel Reepithelialization Mechanism Revealed by in Vitro and in Silico Models. Journal of Cell Biology, 203, 691-709. https://doi.org/10.1083/jcb.201212020
[19]
Marshall, D., Hardman, M.J., Nield, K.M. and Byrne, C. (2001) Differentially Expressed Late Constituents of the Epidermal Cornified Envelope. Proceedings of the National Academy of Sciences of the United States of America, 98, 13031-13036. https://doi.org/10.1073/pnas.231489198
[20]
Niehues, H., van Vlijmen-Willems, I.M.J.J., Bergboer, J.G.M., Kersten, F.F.J., Narita, M., Hendriks, W.J.A.J., et al. (2016) Late Cornified Envelope (LCE) Proteins: Distinct Expression Patterns of LCE2 and LCE3 Members Suggest Nonredundant Roles in Human Epidermis and Other Epithelia. British Journal of Dermatology, 174, 795-802. https://doi.org/10.1111/bjd.14284
[21]
Findley, M.K. and Koval, M. (2009) Regulation and Roles for Claudin-Family Tight Junction Proteins. IUBMB Life, 61, 431-437. https://doi.org/10.1002/iub.175
[22]
Boury-Jamot, M., Sougrat, R., Tailhardat, M., Varlet, B.L., Bonté, F., Dumas, M., et al. (2006) Expression and Function of Aquaporins in Human Skin: Is Aquaporin-3 Just a Glycerol Transporter? Biochimica et Biophysica Acta (BBA)—Biomembranes, 1758, 1034-1042. https://doi.org/10.1016/j.bbamem.2006.06.013
[23]
Boury-Jamot, M., Daraspe, J., Bonté, F., Perrier, E., Schnebert, S., Dumas, M., et al. (2009) Skin Aquaporins: Function in Hydration, Wound Healing, and Skin Epidermis Homeostasis. In: Beitz, E., Ed., Aquaporins, Springer, 205-217. https://doi.org/10.1007/978-3-540-79885-9_10
[24]
Ikarashi, N., Kon, R., Kaneko, M., Mizukami, N., Kusunoki, Y. and Sugiyama, K. (2017) Relationship between Aging-Related Skin Dryness and Aquaporins. International Journal of Molecular Sciences, 18, Article 1559. https://doi.org/10.3390/ijms18071559
[25]
Hara-Chikuma, M. and Verkman, A.S. (2008) Aquaporin-3 Facilitates Epidermal Cell Migration and Proliferation during Wound Healing. Journal of Molecular Medicine, 86, 221-231. https://doi.org/10.1007/s00109-007-0272-4
[26]
Sebastian, R., Chau, E., Fillmore, P., Matthews, J., Price, L.A., Sidhaye, V., et al. (2015) Epidermal Aquaporin-3 Is Increased in the Cutaneous Burn Wound. Burns, 41, 843-847. https://doi.org/10.1016/j.burns.2014.10.033
[27]
Sugiyama, Y., Ota, Y., Hara, M. and Inoue, S. (2001) Osmotic Stress Up-Regulates Aquaporin-3 Gene Expression in Cultured Human Keratinocytes. Biochimica et Biophysica Acta (BBA)—Gene Structure and Expression, 1522, 82-88. https://doi.org/10.1016/s0167-4781(01)00320-7
[28]
Seleit, I., Bakry, O.A., El Rebey, H.S., El-Akabawy, G. and Hamza, G. (2017) Is Aquaporin-3 a Determinant Factor of Intrinsic and Extrinsic Aging? An Immunohistochemical and Morphometric Study. Applied Immunohistochemistry & Molecular Morphology, 25, 49-57. https://doi.org/10.1097/pai.0000000000000265
[29]
Cao, C., Wan, S., Jiang, Q., Amaral, A., Lu, S., Hu, G., et al. (2008) All-Trans Retinoic Acid Attenuates Ultraviolet Radiation-Induced Down-Regulation of Aquaporin-3 and Water Permeability in Human Keratinocytes. Journal of Cellular Physiology, 215, 506-516. https://doi.org/10.1002/jcp.21336
[30]
Dinkova-Kostova, A.T. and Talalay, P. (2010) NAD(P)H:Quinone Acceptor Oxidoreductase 1 (NQO1), a Multifunctional Antioxidant Enzyme and Exceptionally Versatile Cytoprotector. Archives of Biochemistry and Biophysics, 501, 116-123. https://doi.org/10.1016/j.abb.2010.03.019
[31]
Yamaguchi, Y., Hearing, V.J., Maeda, A. and Morita, A. (2010) NADPH:Quinone Oxidoreductase-1 as a New Regulatory Enzyme That Increases Melanin Synthesis. Journal of Investigative Dermatology, 130, 645-647. https://doi.org/10.1038/jid.2009.378
[32]
Patrick, B.A., Gong, X. and Jaiswal, A.K. (2011) Disruption of NAD(P)H:Quinone Oxidoreductase 1 Gene in Mice Leads to 20S Proteasomal Degradation of p63 Resulting in Thinning of Epithelium and Chemical-Induced Skin Cancer. Oncogene, 30, 1098-1107. https://doi.org/10.1038/onc.2010.491
[33]
Choi, T., Sohn, K., Kim, J., Kim, S., Kim, C., Hwang, J., et al. (2010) Impact of NAD(P)H:Quinone Oxidoreductase-1 on Pigmentation. Journal of Investigative Dermatology, 130, 784-792. https://doi.org/10.1038/jid.2009.280
[34]
Moradi, A., Shafiq, F., Robison, T., Colvan, L., Poehler, J. and Widgerow, A.D. (2024) multicenter Evaluation of a Topical Antioxidant Serum. Journal of Cosmetic Dermatology, 23, 145-153. https://doi.org/10.1111/jocd.16067
[35]
Ferrara, F., Yan, X., Pecorelli, A., Guiotto, A., Colella, S., Pasqui, A., et al. (2024) Combined Exposure to UV and PM Affect Skin Oxinflammatory Responses and It Is Prevented by Antioxidant Mix Topical Application: Evidences from Clinical Study. Journal of Cosmetic Dermatology, 23, 2644-2656. https://doi.org/10.1111/jocd.16321
[36]
Frei, B., Kim, M.C. and Ames, B.N. (1990) Ubiquinol-10 Is an Effective Lipid-Soluble Antioxidant at Physiological Concentrations. Proceedings of the National Academy of Sciences of the United States of America, 87, 4879-4883. https://doi.org/10.1073/pnas.87.12.4879
[37]
Negre-Salvayre, A. and Salvayre, R. (2022) Post-Translational Modifications Evoked by Reactive Carbonyl Species in Ultraviolet-A-Exposed Skin: Implication in Fibroblast Senescence and Skin Photoaging. Antioxidants, 11, Article 2281. https://doi.org/10.3390/antiox11112281
