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Pharmacy Information 2026
哺乳动物CHO细胞重组抗体表达的研究进展
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Abstract:
重组抗体药物在医药市场中的占比不断增大。哺乳动物细胞表达系统相比原核和真核表达系统能够高效表达重组抗体并进行近似人源蛋白的翻译后修饰，使重组抗体具有更好的活性及稳定性，因此被广泛用于重组抗体的生产，其中以中国仓鼠卵巢细胞(Chinese Hamster Ovary, CHO)用途最为广泛。基于原有的CHO细胞表达平台，抗体表达产量和质量并不一致。文章聚焦于载体设计、基因整合技术、糖基化修饰、筛选放大策略以及工艺优化等方面的进展并进行综述，旨在探究CHO细胞表达平台面临的目的基因靶向整合效率低、高产克隆筛选繁重、工业放大产物质量不可控等不足，为进一步促进CHO细胞表达系统的效率与一致性提供研究思路。
The proportion of recombinant antibody drugs in the pharmaceutical market is constantly increasing. Compared with prokaryotic and eukaryotic expression systems, mammalian cell expression systems can efficiently express recombinant antibodies and perform post-translational modifications similar to human proteins, endowing recombinant antibodies with better activity and stability. Therefore, they are widely used in the production of recombinant antibodies, among which Chinese hamster ovary cells are the most widely used. Based on the existing CHO cell expression platform, the yield and quality of antibody expression are inconsistent. This article reviews the progress in vector design, gene integration technology, screening and scaling-up strategies, and process optimization, aiming to explore the shortcomings of the CHO cell expression platform, such as low targeting integration efficiency of target genes, heavy workload in screening high-yield clones, and uncontrollable product quality during industrial scaling-up, and to provide research ideas for further improving the efficiency and consistency of the CHO cell expression system.
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