Wheat insoluble arabinoxylan was converted to oligosaccharides carrying feruloyl substituents using the combinatorial enzyme technology. The feruloyl oligos (FOS) were isolated by preparative chromatography, and the active fractions were pooled, and demonstrated to exhibit both antimicrobial and antioxidant activities. The FOS was shown to possess antioxidant activity against oxygen radicals. The Trolox equivalent antioxidant capacity of FOS determined by oxygen radical antioxidant capacity (ORAC) assay averaged 48.2 mM TE for 0.1% solution. The FOS constituted a product with dual functions on health effects.
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