|
|
Pharmacy Information 2026
基于UPLC-MS/MS技术及生物信息学探究藤三七抗胃溃疡的物质基础及作用机制
|
Abstract:
目的：明确藤三七抗胃溃疡(GU)的药效物质及其作用机制。方法：UPLC-Q-TOF-MS鉴定藤三七水提液成分，在TCMSP、GeneCards筛选活性成分与疾病靶点，Venny取交集后构建PPI网络，Cytoscape拓扑分析获取核心靶点。DAVID进行GO与KEGG富集。结果：共鉴定出藤三七成分160种，其中40个符合OB > 30%、DL > 0.18，映射239个GU相关靶点。TNF、IL-6、AKT1、TP53、IL-1β为度值前五位核心靶点；IL-17与TNF信号通路富集最显著。结论：藤三七通过多成分协同调控IL-17/TNF通路，抑制炎症与氧化应激发挥抗胃溃疡作用。
Objective: To identify the bioactive constituents and potential mechanisms of Anredera cordifolia (Ten.) Sreenis in the treatment of gastric ulcer (GU). Methods: Components of the aqueous extract of Anredera cordifolia (Ten.) Sreenis were characterized by UPLC-Q-TOF-MS. Putative active ingredients and GU-related targets were retrieved from TCMSP and GeneCards, respectively. Intersection targets were imported into a Protein-Protein Interaction (PPI) network constructed in Cytoscape. Topological analysis was applied to screen core targets. GO and KEGG enrichment analyses were performed with DAVID. Results: A total of 160 constituents were identified, 40 of which met the criteria of Oral Bioavailability (OB) > 30% and Drug-Likeness (DL) > 0.18. These compounds mapped to 239 GU-associated targets. The top five hub genes ranked by degree were TNF, IL-6, AKT1, TP53 and IL-1β. The IL-17 and TNF signaling pathways were the most significantly enriched. Conclusion: Anredera cordifolia (Ten.) Sreenis exerts anti-gastric-ulcer effects through multi-component synergy that modulates the IL-17/TNF pathway, thereby suppressing inflammation and oxidative stress.
| [1] | Bi, W., Man, H.B. and Man, M.Q. (2014) Efficacy and Safety of Herbal Medicines in Treating Gastric Ulcer: A Review. World Journal of Gastroenterology, 20, 17020-17028. https://doi.org/10.3748/wjg.v20.i45.17020 |
| [2] | Guo, Y., Du, Y., Xie, L., Pu, Y., Yuan, J., Wang, Z., et al. (2020) Effects of Paeonol and Gastroretention Tablets of Paeonol on Experimental Gastric Ulcers and Intestinal Flora in Rats. Inflammation, 43, 2178-2190. https://doi.org/10.1007/s10753-020-01285-y |
| [3] | Gugliandolo, E., Cordaro, M., Fusco, R., Peritore, A.F., Siracusa, R., Genovese, T., et al. (2021) Protective Effect of Snail Secretion Filtrate against Ethanol-Induced Gastric Ulcer in Mice. Scientific Reports, 11, Article No. 3638. https://doi.org/10.1038/s41598-021-83170-8 |
| [4] | Carlotto, J., Maria-Ferreira, D., de Souza, L.M., da Luz, B.B., Dallazen, J.L., de Paula Werner, M.F., et al. (2019) A Polysaccharide Fraction from “Ipê-Roxo” (Handroanthus heptaphyllus) Leaves with Gastroprotective Activity. Carbohydrate Polymers, 226, Article ID: 115239. https://doi.org/10.1016/j.carbpol.2019.115239 |
| [5] | Malfertheiner, P., Kandulski, A. and Venerito, M. (2017) Proton-Pump Inhibitors: Understanding the Complications and Risks. Nature Reviews Gastroenterology & Hepatology, 14, 697-710. https://doi.org/10.1038/nrgastro.2017.117 |
| [6] | 杨思雨. 藤三七的研究综述[J]. 山西林业科技, 2015, 44(3): 40-43. |
| [7] | 王丽君, 樊悦, 谭洋, 等. 藤三七的研究进展[J]. 湖南中医药大学学报, 2018, 38(3): 345-351. |
| [8] | Sukrama, D.M., Wihandani, D.M. and Manuaba, A.P. (2017) Topical Binahong (Anredera cordifolia) Leaf Extract Increases Interleukin-6 and VEGF (Vascular Endothelial Growth Factor) during Burn Wound Healing in Wistar Rats Infected with Pseudomonas Aeruginosa. Biology and Medicine, 9, Article ID: 100036. https://doi.org/10.4172/0974-8369.1000369 |
| [9] | 问慧娟, 李玉巧, 崔玉英, 韩璇. 白藜芦醇对大鼠应激性胃溃疡的保护作用[J]. 中国医院药学杂志, 2014, 34(1): 15-18. |
| [10] | 李雅玲, 冯威, 陈航宇, 等. 齐墩果酸药理作用研究[J]. 生物技术世界, 2015(4): 125, 127. |
| [11] | Albalawi, M. and Khateeb, S. (2025) Development of Glycyrrhizic Acid Nanoparticles for Modulating Gastric Ulcer Healing: A Comparative in Vivo Study Targeting Oxidative Stress and Inflammatory Pathways. Antioxidants, 14, Article 990. https://doi.org/10.3390/antiox14080990 |
| [12] | 王福文, 李杰, 胡志力, 等. 葛根素对大鼠应激性胃黏膜损伤的保护作用[J]. 中国中药杂志, 2006(6): 504-506. |
| [13] | Tourani, M., Habibzadeh, M., Karkhah, A., Shokri-Shirvani, J., Barari, L. and Nouri, H.R. (2018) Association of TNF-α but Not Il-1β Levels with the Presence of Helicobacter pylori Infection Increased the Risk of Peptic Ulcer Development. Cytokine, 110, 232-236. https://doi.org/10.1016/j.cyto.2018.01.003 |
| [14] | Milic, L., Karamarkovic, A., Popadic, D., Sijacki, A., Grigorov, I., Milosevic, E., et al. (2019) Altered Cytokine Expression in Helicobacter pylori Infected Patients with Bleeding Duodenal Ulcer. BMC Research Notes, 12, Article No. 278. https://doi.org/10.1186/s13104-019-4310-4 |
| [15] | Yin, T., Zhang, H., Liu, X., Wei, D., Ren, C., Cui, L., et al. (2024) Elucidating the Anti-Hypertensive Mechanisms of Uncaria rhynchophylla-Alisma plantago-Aquatica L: An Integrated Network Pharmacology, Cluster Analysis, and Molecular Docking Approach. Frontiers in Chemistry, 12, Article 1356458. https://doi.org/10.3389/fchem.2024.1356458 |
| [16] | Zhou, B., Qian, Z., Li, Q., Gao, Y. and Li, M. (2022) Assessment of Pulmonary Infectious Disease Treatment with Mongolian Medicine Formulae Based on Data Mining, Network Pharmacology and Molecular Docking. Chinese Herbal Medicines, 14, 432-448. https://doi.org/10.1016/j.chmed.2022.07.001 |
| [17] | Verma, S. and Kumar, V.L. (2016) Attenuation of Gastric Mucosal Damage by Artesunate in Rat: Modulation of Oxidative Stress and NFκB Mediated Signaling. Chemico-Biological Interactions, 257, 46-53. https://doi.org/10.1016/j.cbi.2016.07.027 |
| [18] | Hou, Y., Wang, W., Ye, J., Sun, L., Zhou, S., Zheng, Q., et al. (2025) The Crucial Role of Neutrophil Extracellular Traps and IL-17 Signaling in Indomethacin-Induced Gastric Injury in Mice. Scientific Reports, 15, Article No. 12109. https://doi.org/10.1038/s41598-025-95880-4 |
| [19] | Bagheri, N., Razavi, A., Pourgheysari, B., Azadegan-Dehkordi, F., Rahimian, G., Pirayesh, A., et al. (2018) Up-Regulated Th17 Cell Function Is Associated with Increased Peptic Ulcer Disease in Helicobacter pylori-Infection. Infection, Genetics and Evolution, 60, 117-125. https://doi.org/10.1016/j.meegid.2018.02.020 |
| [20] | Zheng, Y., Liu, Y., Li, H., Wang, X., Zhang, M., Shen, X., et al. (2021) Novel Insights into the Immune Regulatory Effects of Megalobrama amblycephala Intelectin on the Phagocytosis and Killing Activity of Macrophages. Molecular Immunology, 137, 145-154. https://doi.org/10.1016/j.molimm.2021.06.008 |
| [21] | Gao, J., Liu, Y., Chen, J., Tong, C., Wang, Q. and Piao, Y. (2022) Curcumin Treatment Attenuates Cisplatin-Induced Gastric Mucosal Inflammation and Apoptosis through the NF-κB and MAPKs Signaling Pathway. Human & Experimental Toxicology, 41, 1-11. https://doi.org/10.1177/09603271221128738 |
| [22] | da Silva Junior, I.F., Balogun, S.O., de Oliveira, R.G., Damazo, A.S. and Martins, D.T.D.O. (2016) Piper umbellatum L.: A Medicinal Plant with Gastric-Ulcer Protective and Ulcer Healing Effects in Experimental Rodent Models. Journal of Ethnopharmacology, 192, 123-131. https://doi.org/10.1016/j.jep.2016.07.011 |
| [23] | Wang, C., Wu, Y., Gong, B., Mou, J., Cheng, X., Zhang, L., et al. (2025) Agarwood Chromone Alleviates Gastric Ulcers by Inhibiting the NF-κB and Caspase Pathways Based on Network Pharmacology and Molecular Docking. Pharmaceuticals, 18, Article 514. https://doi.org/10.3390/ph18040514 |
| [24] | Liu, J., Lin, H., Yuan, L., Wang, D., Wang, C., Sun, J., et al. (2021) Protective Effects of Anwulignan against HCL/Ethanol-Induced Acute Gastric Ulcer in Mice. Evidence-Based Complementary and Alternative Medicine, 2021, Article ID: 9998982. https://doi.org/10.1155/2021/9998982 |
| [25] | Gong, H., Han, D., Luo, Z., Zhu, Q., Zhu, X., Liu, S., et al. (2023) Xiangshao Decoction Alleviates Gastric Mucosal Injury through NRF2 Signaling Pathway and Reduces Neuroinflammation in Gastric Ulcer Rats. Phytomedicine, 118, Article ID: 154954. https://doi.org/10.1016/j.phymed.2023.154954 |
