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AggreWellTM800板培养乳腺癌肿瘤球模型及其用于盐酸青藤碱药敏实验
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Abstract:
目的：通过AggreWell板构建乳腺癌肿瘤球模型，并用于盐酸青藤碱对乳腺癌MDA-MB-231细胞的药敏试验。方法：AggreWellTM800板接种MDA-MB-231细胞，构建乳腺癌3D肿瘤球。采用盐酸青藤碱进行药敏试验，CCK-8试剂盒检测。并与2D培养比较。结果：随着加入盐酸青藤碱浓度的升高，MDA-MB-231细胞抑制率逐渐上升，呈剂量依赖性。AggreWell板培养的乳腺癌3D肿瘤球对盐酸青藤碱的药物敏感性显著低于传统2D培养。结论：本实验研究表明，相比于传统2D培养，使用AggreWell板培养的乳腺癌3D肿瘤球对盐酸青藤碱的药敏性显著更低，可能更能反映体内肿瘤的真实情况，在评估抗癌药物方面可能更具优势。
Objective: To construct a breast cancer tumor spheroid model using the AggreWell plate and apply it to the drug sensitivity test of sinomenine hydrochloride on breast cancer MDA-MB-231 cells. Methods: MDA-MB-231 cells were seeded in the AggreWellTM800 plate to form 3D breast cancer tumor spheroid. The sinomenine hydrochloride was used for the drug sensitivity test, and the CCK-8 kit was employed for detection. A comparison was made with 2D culture. Results: As the concentration of sinomenine hydrochloride added increased, the inhibition rate of MDA-MB-231 cells gradually rose, showing a dose-dependent manner. The drug sensitivity of the 3D breast cancer tumor spheroid cultured in the AggreWell plate to sinomenine hydrochloride was significantly lower than that of traditional 2D culture. Conclusion: This experimental study indicates that compared with traditional 2D culture, the 3D breast cancer tumor spheroid cultured in the AggreWell plate has significantly lower drug sensitivity to sinomenine hydrochloride, which may better reflect the real situation of the tumor in the body and may have more advantages in evaluating anti-cancer drugs.
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