|
|
不同规格线纹海马氨基酸构成和降压肽的分析研究
|
Abstract:
线纹海马(Hippocampus erectus)是如今海马养殖业热门品种，因其具有高抗病性、繁殖能力强等优势，逐渐变成了海马养殖业的首选种，具有极高的药用价值和观赏价值。本文以线纹海马为研究对象，通过分析测定不同规格的线纹海马氨基酸构成和海马降压肽对血管紧张素转化酶ACE的抑制率来研究线纹海马制备降压肽的条件。结果表明，高效液相色谱法在料液比为1:15，加酶量为3%，酶解温度为37℃的条件下，酶解时间6 h，商品规格为12 cm以上的线纹海马抑制率最高，制备降压肽效果最好。
The lined seahorse (Hippocampus erectus) is a hot variety in the seahorse breeding industry today. Because of its advantages of high disease resistance and strong reproductive ability, it has gradually become the preferred species in the seahorse breeding industry, with extreme medicinal value and ornamental value. In this paper, the lined seahorse was selected as the research object, and the conditions for the preparation of the lined seahorse antihypertensive peptide were studied by analyzing the amino acid composition of the lined seahorse of different specifications and the inhibitory rate of the hippocampal antihypertensive peptide on ACE. The results showed that under the conditions of a solid-liquid ratio of 1:15, an enzyme dosage of 3%, an enzymatic hydrolysis temperature of 37？C, and an enzymatic hydrolysis time of 6 hours, the inhibition rate of linear hippocampus with a commercial specification of 12 cm or more was the highest, and the effect of preparing antihypertensive peptides was the best.
| [1] | 王峰, 丁剑玲, 王有昆, 等. 线纹海马的雌雄个体形态差异分析[J]. 水产研究, 2020, 7(4): 188-197. |
| [2] | 曾涛, 万莉红, 易智慧, 等. 肾素-血管紧张素系统对中枢神经系统疾病的影响[J]. 四川生理科学杂志, 2024, 46(6): 1409-1412. |
| [3] | Oparil, S., Acelajado, M.C., Bakris, G.L., Berlowitz, D.R., Cífková, R., Dominiczak, A.F., et al. (2018) Hypertension. Nature Reviews Disease Primers, 4, 34-47. https://doi.org/10.1038/nrdp.2018.14 |
| [4] | Chen, Q., Liu, X., Wang, T., Huang, C., Zhang, Z., Chen, J., et al. (2024) The Effect of the Hippocampus Erectus Decoction on Improving Osteoporosis in Zebrafish. Aquaculture Reports, 39, Article 102510. https://doi.org/10.1016/j.aqrep.2024.102510 |
| [5] | Chen, L., Wang, X. and Huang, B. (2015) The Genus Hippocampus—A Review on Traditional Medicinal Uses, Chemical Constituents and Pharmacological Properties. Journal of Ethnopharmacology, 162, 104-111. https://doi.org/10.1016/j.jep.2014.12.032 |
| [6] | Wang, J., Ye, X., Su, Z., Zou, P., Pang, J. and Chen, J. (2021) Ace-Inhibitory Peptides from Laminaria japonica and Their Potential Anti-Hypertensive Mechanism. CyTA-Journal of Food, 19, 333-340. https://doi.org/10.1080/19476337.2021.1900923 |
| [7] | Fitzgerald, R.J. and Murray, B.A. (2010) Bioactive Peptides and Lactic Fermentations. International Journal of Dairy Technology, 59, 118-125. https://doi.org/10.1111/j.1471-0307.2006.00250.x |
| [8] | Iwaniak, A., Minkiewicz, P. and Darewicz, M. (2014) Food‐Originating ACE Inhibitors, Including Antihypertensive Peptides, as Preventive Food Components in Blood Pressure Reduction. Comprehensive Reviews in Food Science and Food Safety, 13, 114-134. https://doi.org/10.1111/1541-4337.12051 |
| [9] | Meisel, H. (2004) Multifunctional Peptides Encrypted in Milk Proteins. BioFactors, 21, 55-61. https://doi.org/10.1002/biof.552210111 |
| [10] | 黄莹, 黄晶, 杨晓, 等. PITC柱前衍生-高效液相色谱法测大米肽中的16种氨基酸含量[J]. 食品工业科技, 2023, 44(24): 279-285. |
| [11] | Tian, W., Zhang, C., Zheng, Q., Hu, S., Yan, W., Yue, L., et al. (2024) In Silico Screening of Bioactive Peptides in Stout Beer and Analysis of ACE Inhibitory Activity. Foods, 13, Article 1973. https://doi.org/10.3390/foods13131973 |
| [12] | Ko, S., Kang, N., Kim, E., Kang, M.C., Lee, S., Kang, S., et al. (2012) A Novel Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptide from a Marine Chlorella Ellipsoidea and Its Antihypertensive Effect in Spontaneously Hypertensive Rats. Process Biochemistry, 47, 2005-2011. https://doi.org/10.1016/j.procbio.2012.07.015 |
| [13] | Qu, W., Ma, H., Jia, J., He, R., Luo, L. and Pan, Z. (2012) Enzymolysis Kinetics and Activities of ACE Inhibitory Peptides from Wheat Germ Protein Prepared with SFP Ultrasound-Assisted Processing. Ultrasonics Sonochemistry, 19, 1021-1026. https://doi.org/10.1016/j.ultsonch.2012.02.006 |
| [14] | 赵谋明, 赖晨戎, 崔春. 高效液相色谱测定ACE抑制率方法改进的研究[J]. 现代食品科技, 2013, 29(11): 2742-2746. |
| [15] | Cushman, D.W. and Cheung, H.S. (1971) Spectrophotometric Assay and Properties of the Angiotensin-Converting Enzyme of Rabbit Lung. Biochemical Pharmacology, 20, 1637-1648. https://doi.org/10.1016/0006-2952(71)90292-9 |
| [16] | Cao, X., Zhou, H., Xie, J., Zhang, Z., Guo, S., Luo, J., et al. (2025) A Novel Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptide from Wuyi Rock Tea Residue: Preparation, Identification, and Its Potential Molecular Mechanism. LWT, 216, Article 117353. https://doi.org/10.1016/j.lwt.2025.117353 |
| [17] | 杨贵兰, 秦松, 王晓艳, 等. 酶法制备阿拉斯加鳕鱼降压肽的工艺优化及其产物的结构鉴定[J]. 食品工业科技, 2020, 41(5): 163-168, 174. |
| [18] | 廖乾莉, 范小春. 高血压合并心力衰竭一线降压药物治疗的研究进展[J]. 中国医药, 2024, 19(5): 768-772. |
| [19] | 杨思雨, 王苗苗, 陈焕根. 不同地区小龙虾氨基酸含量及挥发性风味物质成分比较研究[J]. 现代农业科技, 2024(8): 129-133. |
| [20] | Feng, B., Zhang, H., Zhang, D., Luo, Y., Yang, H., Lin, J., et al. (2024) Comprehensive Biochemical Analysis and Nutritional Evaluation of Fatty Acid and Amino Acid Profiles in Eight Seahorse Species (Hippocampus Spp.). Heliyon, 10, e33220. https://doi.org/10.1016/j.heliyon.2024.e33220 |
