Isolation and Molecular Characterization of Streptococcusiniae from African Catfish (Clariasgariepinus) from Selected Markets in Federal Capital Territory, Abuja, Nigeria
Fish plays an important role in the human diet. Streptococcusiniae is an opportunistic zoonotic pathogen and causes serious infections in humans, often through transmission of Streptococcusiniae-contaminated fish. The risk of zoonotic transmission to humans highlights the need to evaluate Streptococcusiniae from African catfish (Clariasgariepinus) in the Federal Capital Territory, Abuja. To achieve these goals, four hundred (400) samples of Clariasgariepinus organs namely liver, kidney, intestine, gills, heart and skin were purchased from selected markets (Wuse, Garki, Kado fish market, Gwagwalada, Zuba, Kubwa and Duste Allah). Detection of Streptococcusiniae was carried out using standard method on tryptic soy agar. Isolates were further identified using conventional biochemical tests: Gram’s staining, catalase, oxidase, hemolytic zone on 5% sheep blood agar, hydrolysis of starch and fermentation of mannitol, ribose, xlylose, sorbitol, glucose, inulin, lactose, arginine, arabinose, inositol, raffinose, rhamnose. Forty-three 43 (10.8%) suspected isolates of Streptococci were subjected to species specific polymerase chain reaction for amplification at 377 bp and all of the isolates turned out negative. It is therefore suggested that more investigative studies become necessary to determine the presence of this pathogen in different fish species in Nigeria.
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