This study aimed to determine the feasibility of using the tiletamine-zolazepam-butorphanol-dexmedetomidine (TDex) combination compared to the use of butorphanol-propofol-isoflurane (Iso) for performing electroretinography (ERG) in healthy Belgian Malinois dogs. Six (6) dogs were subjected to different anesthesia protocols: the combination of tiletamine-zolazepam-butorphanol-dexmedetomidine (TDex) or tiletamine-zolazepam via the intramuscular (IM) route, and butorphanol-propofol-isoflurane (Iso) via intravenous route with anesthetic maintenance using isoflurane. Electroretinography was performed using the BPM2005 equipment with focal stimulation and three electrodes: recording, reference, and ground. The examination began 20 minutes after dark adaptation and continued with ambient lights on after a 10-minute light adaptation period. For statistical analysis, results were expressed as Mean ± Standard Error (SE). Data normality was assessed using the Shapiro-Wilk test, followed by the unpaired Student’s t-test for parametric data, and the Mann-Whitney test for non-parametric data. Grubb’s test was used to identify outliers. Significance levels were set at p < 0.05, 0.01, 0.001, and 0.0001. Similarities were observed between groups; however, ERG performed under inhalation anesthesia showed superior amplitude values, reduced implicit times, shorter total examination time, and better anesthetic recovery compared to the TDex combination. The electroretinographic recordings also exhibited less noise in the Iso group. Regarding physiological parameters, the Iso group showed better cardiorespiratory stability compared to the TDex group.
References
[1]
Safatle, A.M.V., Hvenegaard, A.P., Kahvegian, M., Martins, T.L., Shimamura, G.M., Fantoni, D.T., et al. (2010) Padronização do eletrorretinograma de campo total em cães. Pesquisa Veterinária Brasileira, 30, 763-769. https://doi.org/10.1590/s0100-736x2010000900009
[2]
Ekesten, B., Komáromy, A.M., Ofri, R., Petersen-Jones, S.M. and Narfström, K. (2013) Guidelines for Clinical Electroretinography in the Dog: 2012 Update. Documenta Ophthalmologica, 127, 79-87. https://doi.org/10.1007/s10633-013-9388-8
[3]
Sussadee, M., Phavaphutanon, J., Kornkaewrat, K. and Thayananuphat, A. (2015) Normal Clinical Electroretinography Parameters for Poodle, Labrador Retriever, Thai Ridgeback, and Thai Bangkaew. Journal of Veterinary Science, 16, 67-74. https://doi.org/10.4142/jvs.2015.16.1.67
[4]
Nedev, V.S. and Simeonova, G.P. (2018) Normal Clinical Electroretinography Parameters of Healthy Pharaoh Hounddogs. Advances in Animal and Veterinary Sciences, 6, 81-87. https://doi.org/10.17582/journal.aavs/2018/6.2.81.87
[5]
Freeman, K.S., Good, K.L., Kass, P.H., Park, S.A., Nestorowicz, N. and Ofri, R. (2013) Effects of Chemical Restraint on Electroretinograms Recorded Sequentially in Awake, Sedated, and Anesthetized Dogs. American Journal of Veterinary Research, 74, 1036-1042. https://doi.org/10.2460/ajvr.74.7.1036
[6]
Lin, S., Shiu, W., Liu, P., Cheng, F., Lin, Y. and Wang, W. (2009) The Effects of Different Anesthetic Agents on Short Electroretinography Protocol in Dogs. Journal of Veterinary Medical Science, 71, 763-768. https://doi.org/10.1292/jvms.71.763
[7]
Jeong, M.B., Narfström, K., Park, S.A., Chae, J.M. and Seo, K.M. (2009) Comparison of the Effects of Three Different Combinations of General Anesthetics on the Electroretinogram of Dogs. Documenta Ophthalmologica, 119, 79-88. https://doi.org/10.1007/s10633-009-9173-x
[8]
Kommonen, B., Hyvätti, E. and Dawson, W.W. (2007) Propofol Modulates Inner Retina Function in Beagles. Veterinary Ophthalmology, 10, 76-80. https://doi.org/10.1111/j.1463-5224.2007.00512.x
[9]
Tranquilli, J.W., Thurmon, J.C. and Grimm, K.A. (2013) Lumb & Jones: Anestesiologia e analgesia veterinária. Roca.
[10]
Norman, S.A., DiGiacomo, R.F., Gulland, F.M.D., Meschke, J.S. and Lowry, M.S. (2008) Risk Factors for an Outbreak of Leptospirosis in California Sea Lions (Zalophus californianus) in California, 2004. Journal of Wildlife Diseases, 44, 837-844. https://doi.org/10.7589/0090-3558-44.4.837
[11]
Mazzaferro, E. and Wagner, A.E. (2001) Hypotension during Anesthesia in Dogs and Cats: Recognition, Causes, and Treatment. Compendium on Continuing Education for the Practicing Veterinarian, 23, 728-737.
[12]
Granholm, M., McKusick, B.C., Westerholm, F.C. and Aspegrén, J.C. (2006) Evaluation of the Clinical Efficacy and Safety of Dexmedetomidine or Medetomidine in Cats and Their Reversal with Atipamezole. Veterinary Anaesthesia and Analgesia, 33, 214-223. https://doi.org/10.1111/j.1467-2995.2005.00259.x
[13]
Hubbell, J.A.E. and Muir, W.W. (2010) Antagonism of Detomidine Sedation in the Horse Using Intravenous Tolazoline or Atipamezole. Equine Veterinary Journal, 38, 238-241. https://doi.org/10.2746/042516406776866408
[14]
Carregaro, A.B., Gerardi, P.M., Mujalli, M.C.L.C. and Martins, F.S. (2006) Uso de atracúrio para o bloqueio da musculatura extrínseca do bulbo ocular em cães submetidos à anestesia inalatória sob ventilação espontânea. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 58, 1057-1063. https://doi.org/10.1590/s0102-09352006000600013
[15]
Nair, G., Kim, M., Nagaoka, T., Olson, D.E., Thulé, P.M., Pardue, M.T., et al. (2011) Effects of Common Anesthetics on Eye Movement and Electroretinogram. Documenta Ophthalmologica, 122, 163-176. https://doi.org/10.1007/s10633-011-9271-4
[16]
Oliver, J.A.C., Clark, L., Corletto, F. and Gould, D.J. (2010) A Comparison of Anesthetic Complications between Diabetic and Nondiabetic Dogs Undergoing Phacoemulsification Cataract Surgery: A Retrospective Study. Veterinary Ophthalmology, 13, 244-250. https://doi.org/10.1111/j.1463-5224.2010.00793.x
