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ACAT1 rs10913733基因多态性与非酒精性脂肪性肝病发病风险的相关性研究
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Abstract:
目的：在中国青岛地区人群中，探讨ACAT1 rs10913733位点基因多态性与非酒精性脂肪性肝病发病风险的相关性。方法：随机纳入2022年06月~2023年06月就诊于青岛市市立医院的289名不同性别、年龄的受试者，其中NAFLD患者197人、健康对照者92人。采集受试者一般临床资料，并检测血液生化学指标。采用PCR以及MALDI-TOF质谱分析技术检测ACAT1 rs10913733基因型。采用SPSS 26.0软件进行统计学分析。结果：ACAT1 rs10913733位点基因型(GG, GT, TT)及等位基因分布在NAFLD组和健康对照组之间无明显差异(P > 0.05)。应用非条件logistics回归分析显示，ACAT1 rs10913733位点基因型及等位基因分布频率与NAFLD发病风险无相关性(P > 0.05)。在所有受试者及NAFLD组患者中，T等位基因非携带者有着更高的TC水平(P < 0.05)。结论：在中国青岛部分人群中，ACAT1 rs10913733多态性与NAFLD发病风险无显著相关性。在所有受试者及NAFLD组中ACAT1 rs10913733的T等位基因非携带均与血胆固醇水平升高有关。
Objective: To investigate the relationship between the gene polymorphism of ACAT1rs109137 at position 33 and the risk of non-alcoholic fatty liver disease (NAFLD) in a population in Qingdao, China. Methods: A total of 289 subjects of different genders and ages were randomly enrolled from June 2022 to June 2023 at the Qingdao Municipal Hospital. Among them, 197 were NAFLD patients and 92 were healthy controls. All subjects’ general clinical data were collected, and blood biochemical indicators were measured. Total DNA was extracted, and the ACAT1 rs109137 polymorphism was detected by PCR and MALDI-TOF mass spectrometry. Statistical analyses were conducted using SPSS 26.0 software. Results: There was no significant difference in the genotype and allele frequency of the ACAT1 rs10913733 locus (GG, GT, and TT) between the NAFLD group and the healthy control group (P > 0.05). Non-conditional logistic regression analysis showed that the ACAT1 rs10913733 locus genotype and allele frequency were not associated with the risk of NAFLD (P > 0.05). In all subjects and NAFLD patients, T allele non-carriers had higher TC levels (P < 0.05). Conclusion: In a subset of the population in Qingdao, China, the ACAT1 rs10913733 polymorphism is not significantly associated with the risk of NAFLD. In all subjects and NAFLD patients, being a non-carrier of the T allele of ACAT1 rs10913733 is associated with higher blood cholesterol levels.
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