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聚集诱导发光型光敏剂合成及活性评价
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Abstract:
目的：本文基于四苯乙烯母核结构合成了光敏剂分子OMe-DiS-DiPy，并对其光动力治疗活性进行了探究。方法：通过紫外–可见光光谱及荧光谱对光敏剂的基本光学性质及光动力活性进行了测定。通过DCFH-DA荧光探针，ABDA探针以及DHR123荧光探针分别对光敏剂在溶液中的总ROS，1O2及O2？˙生成进行测定。结果：所合成的光敏剂在400~500 nm具有较强的吸收，在550~750 nm具有较强的荧光发射，在溶液中能够产生ROS。结论：综上所述，本文所合成的光敏剂具有较好的ROS生成能力并通过I型途径及II型途径产生ROS。
Objective: In this study, we designed and synthesized a photosensitizer molecule (OMe-DiS-DiPy) based on a tetraphenylethylene (TPE) scaffold, and systematically evaluated its photodynamic therapy (PDT) efficacy. Methods: The optical properties and photodynamic activity were characterized using UV-Vis and fluorescence spectroscopy. The total ROS, 1O2 and O2？˙ production were measured by DCFH-DA, ABDA and DHR123 probes, respectively. Results: The synthesized photosensitizer exhibited strong absorption at 400~500 nm and intense fluorescence emission at 550~750 nm. ROS production was observed in solution. Conclution: In conclusion, the photosensitizers synthesized in this work have good ROS generation ability and produce ROS through the type I and type II pathways.
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