方斑东风螺细菌性病害病原菌的分离鉴定及人工感染研究
Isolation, Identification of Pathogenic Bacteria of Bacterial Diseases in Babylonia areolata and Studies on Artificial Infection

方斑东风螺是我国东南沿海重要的经济贝类，然而近年来细菌性病害严重制约其养殖产业发展。本研究旨在分离鉴定方斑东风螺细菌性病害的病原菌，并通过人工感染实验探究其致病特性。从患病螺体中分离优势菌，进行纯化、鉴定及药敏实验，同时开展人工感染实验评估病原菌致病性，建立剂量–效应关系。结果显示，发病螺体内弧菌载量显著升高，分离菌株对多种抗生素耐药。人工感染实验表明，哈维氏弧菌等病原菌的致病性与接种剂量呈正相关。本研究为方斑东风螺细菌性病害的防控提供了重要的理论依据。
Babylonia areolata is an important economic shellfish along the southeast coast of China. However, in recent years, bacterial diseases have severely restricted the development of its aquaculture industry. This study aims to isolate and identify the pathogenic bacteria of bacterial diseases in Babylonia areolata, and explore their pathogenic characteristics through artificial infection experiments. Dominant bacteria were isolated from diseased snails, and purification, identification, and drug sensitivity tests were carried out. At the same time, artificial infection experiments were conducted to evaluate the pathogenicity of the pathogenic bacteria and establish a dose - response relationship. The results showed that the Vibrio load in diseased snails increased significantly, and the isolated strains were resistant to multiple antibiotics. Artificial infection experiments indicated that the pathogenicity of pathogenic bacteria such as Vibrio harveyi was positively correlated with the inoculation dose. This study provides an important theoretical basis for the prevention and control of bacterial diseases in Babylonia areolata.