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浆液性卵巢癌中tiRNA-His-GTG-001的表达和功能研究
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Abstract:
目的：tRNA衍生小RNA (tsRNAs)是一种新型的非编码RNA。本研究旨在探讨tiRNA-His-GTG-001在卵巢浆液性癌(SOC)中的表达、临床病理意义和功能。方法：通过tsRNA芯片检测SOC和邻近正常组织中差异性表达的tsRNAs，并通过qRT-PCR验证tiRNA-His-GTG-001在63例SOC中的表达。细胞克隆形成实验和CCK-8法检测细胞增殖与生长；Transwell实验检测细胞迁移能力。结果：tRF-tiRNA-His-GTG-001在63例SOC中的表达水平上调；tiRNA-His-GTG-001表达与肿瘤直径和病理分级密切相关。过表达tiRNA-His-GTG-001后，细胞增殖活性升高、克隆数目增多；降低tiRNA-His-GTG-001表达后，细胞增殖活性下降、克隆数目减少。在Transwell迁移实验中，tiRNA-His-GTG-001表达与细胞迁移无关。结论：在SOC中tRF-tiRNA-His-GTG-001表达水平上调，是一种新的促癌基因。tRF-tiRNA-His-GTG-001促进卵巢癌细胞的增殖和生长，而与迁移无关。本研究为SOC增殖生长提供了新的标志物，并可能为SOC的诊治提供了潜在的策略。
Objective: tRNA-derived small RNAs (tsRNAs) is a novel non-coding RNA. This study is to investigate the expression, clinicopathological significance and function of tiRNA-His-GTG-001 in serous ovarian cancer (SOC). Methods: The differentially expressed tsRNAs in SOC and adjacent normal tissues were detected by tsRNA chip, and the expression of tiRNA-His-GTG-001 in SOC was verified by qRT-PCR in 63 cases. Cell cloning formation assay and CCK-8 assay were used to detect cell proliferation and growth. Transwell assay was used to detect cell migration ability. Results: The expression of tiRNA-His-GTG-001 was up-regulated in 63 SOC. The expression of tiRNA-His-GTG-001 was closely related to tumor diameter and pathological grade. After overexpression of tiRNA-His-GTG-001, cell proliferation activity and clone number increased. After the expression of tiRNA-His-GTG-001 was decreased, the cell proliferation activity and clone number decreased. In Transwell migration assay, tiRNA-His-GTG-001 expression was not associated with cell migration. Conclusions: tiRNA-His-GTG-001 is up-regulated in SOC, suggesting that tiRNA-His-GTG-001 is a novel oncogene. tiRNA-His-GTG-001 promoted ovarian cancer cell proliferation and growth, but not migration. This study provides a new marker for the proliferation and growth of SOC, and may provide a potential strategy for the diagnosis and treatment of SOC.
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