With the development of economic globalization and information technology, knowledge employees are increasingly becoming a key factor in the competitiveness of enterprises. Thriving at work as a positive experience formed by employees’ thriving at work can effectively realize employees’ self-growth and sustainable development of the enterprise. Combined with the social embeddedness of thriving at work, this paper explores the effect of meaning of work on thriving at work of knowledge employees based on the data of 176 questionnaires from enterprise W. Difference sequence is selected as the moderating variable. The results showed that meaning of work had a significant positive effect on both sense of learning and sense of vitality of thriving at work, and the facilitating effect on sense of vitality was stronger; difference sequence atmosphere negatively moderated the relationship between meaning of work and the dimensions of sense of learning of thriving at work.
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