Introduction: Bacteraemia is a serious infection that is responsible for high morbidity and mortality worldwide through the use of central or peripheral venous catheters. Material and Method: This was a prospective descriptive cross-sectional study of patients hospitalized at the Niamey General Reference Hospital. Patient blood was collected during a thermal peak and inoculated into FN PLUS, FA PLUS and PF PLUS blood culture bottles according to patient age, and sent to the Microbiology laboratory for analysis. These flasks were introduced into the BactAlerT 3D at 37？C for incubation. Positive flasks were subcultured onto appropriate media for bacterial growth and identification, and tested for antibiotic sensitivity at 0.5 MacFarland by the Kurbi Bauer method. Data were entered into Microsoft Excel and analyzed using SPSS version 16 software. Result: A total of 250 patients were included in our study. The mean age of the patients was 45 ± 17 years. The prevalence of bacteremia among patients was 14%. The bacteria encountered were Staphylococcus aureus (31.43%), Escherichia coli (22.86%), Klebsiella pneumoniae (11.43%) and other bacterial species (8.57%). Bacteremia were more common in men, and patients hospitalized in the Neurology, Internal Medicine and Emergency Departments had higher bacteremia levels, at 4.4%, 2.8% and 2.4% respectively. Staphylococcus aureus strains were sensitive to oxacillin, norfloxacin, levofloxacin (18.18%), erythromycin (72.73%), gentamicin and tetracycline (36, 36%) Escherichia coli strains showed sensitivity to amoxicillin (12.50%), amoxicillin/clavulanic acid, piperacillin, ciprofloxacin and amikacin (25%). No Escherichia coli strains were sensitive to 3rd-generation cephalosporins, but ertapenem and imipenem were sensitive (100%) to all strains. Conclusion: Bacteremia is common, especially in patients with central venous catheters, with a high frequency of Staphylococcus aureus and Escherichia coli, antibiotic resistance to these isolates is very high in hospitalized patients.
References
[1]
Holmes, C.L., Anderson, M.T., Mobley, H.L.T. and Bachman, M.A. (2021) Pathogenesis of Gram-Negative Bacteremia. Clinical Microbiology Reviews, 34, e00234-20. https://doi.org/10.1128/cmr.00234-20
[2]
Lamy, B., Sundqvist, M. and Idelevich, E.A. (2020) Bloodstream Infections—Standard and Progress in Pathogen Diagnostics. Clinical Microbiology and Infection, 26, 142-150. https://doi.org/10.1016/j.cmi.2019.11.017
[3]
Verway, M., Brown, K.A., Marchand-Austin, A., Diong, C., Lee, S., Langford, B., et al. (2022) Prevalence and Mortality Associated with Bloodstream Organisms: A Population-Wide Retrospective Cohort Study. Journal of Clinical Microbiology, 60, e02429-21. https://doi.org/10.1128/jcm.02429-21
[4]
CDC (2020) Sepsis Data and Reports: Clinical Information. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, Atlanta, GA.
[5]
Iliyasu, G., Dayyab, F.M., Aminu, A., Halilu, S., Abubakar, S., Inuwa, S., et al. (2019) Epidemiology and Microbiology of Bacterial Bloodstream Infection in a Tertiary Hospital in Nigeria. Infectious Diseases in Clinical Practice, 28, 16-21. https://doi.org/10.1097/ipc.0000000000000783
[6]
Andersen, C.T., Langendorf, C., Garba, S., Sayinzonga-Makombe, N., Mambula, C., Mouniaman, I., et al. (2022) Risk of Community-and Hospital-Acquired Bacteremia and Profile of Antibiotic Resistance in Children Hospitalized with Severe Acute Malnutrition in Niger. International Journal of Infectious Diseases, 119, 163-171. https://doi.org/10.1016/j.ijid.2022.03.047
[7]
Cissoko, Y. and Al, E. (2023) Méta analyse de la sensibilité aux antibiotiques des bactéries isolées dans les hémocultures au Mali de 2000-2020. Revue Malienned’Infectiologie et de Microbiologie, 18, 49-54. https://doi.org/10.53597/remim.v18i1.2630
[8]
Riu, M., Chiarello, P., Terradas, R., Sala, M., Castells, X., Knobel, H., et al. (2016) Impacto económico de las bacteriemias nosocomiales. Comparación de tres metodologías de cálculo. EnfermedadesInfecciosas y MicrobiologíaClínica, 34, 620-625. https://doi.org/10.1016/j.eimc.2015.09.007
[9]
Canzoneri, C.N., Akhavan, B.J., Tosur, Z., Andrade, P.E.A. and Aisenberg, G.M. (2017) Follow-up Blood Cultures in Gram-Negative Bacteremia: Are They Needed? Clinical Infectious Diseases, 65, 1776-1779. https://doi.org/10.1093/cid/cix648
[10]
Marchel, H. and Wróblewska, M. (2021) Bloodstream Infections—Etiology and Current Microbiological Diagnostics. Polish Annals of Medicine, 29, 83-87. https://doi.org/10.29089/2021.21.00193
[11]
Rodríguez-Baño, J., Gutiérrez-Gutiérrez, B. and Kahlmeter, G. (2019) Antibiotics for Ceftriaxone-Resistant Gram-Negative Bacterial Bloodstream Infections. JAMA, 321, 612-613. https://doi.org/10.1001/jama.2018.19345
[12]
Le Doare, K., Bielicki, J., Heath, P.T. and Sharland, M. (2014) Systematic Review of Antibiotic Resistance Rates among Gram-Negative Bacteria in Children with Sepsis in Resource-Limited Countries. Journal of the Pediatric Infectious Diseases Society, 4, 11-20. https://doi.org/10.1093/jpids/piu014
[13]
Strich, J.R., Heil, E.L. and Masur, H. (2020) Considerations for Empiric Antimicrobial Therapy in Sepsis and Septic Shock in an Era of Antimicrobial Resistance. The Journal of Infectious Diseases, 222, S119-S131. https://doi.org/10.1093/infdis/jiaa221
[14]
Igunma, J.A., Awopeju, T.A.O., Olonipile, F.A. and Umasoye, A. (2021) Investigation of Gram-Negative Bacilli Bacteraemia in a Tertiary Hospital in Nigeria: Epidemiology and Antimicrobial Susceptibility Pattern. International Journal of Enteric Pathogens, 9, 31-36. https://doi.org/10.34172/ijep.2021.07
[15]
Diekema, D.J., Hsueh, P., Mendes, R.E., Pfaller, M.A., Rolston, K.V., Sader, H.S., et al. (2019) The Microbiology of Bloodstream Infection: 20-Year Trends from the SENTRY Antimicrobial Surveillance Program. Antimicrobial Agents and Chemotherapy, 63, e00355-19. https://doi.org/10.1128/aac.00355-19
[16]
Torres, I., Huntley, D., Tormo, M., Calabuig, M., Hernández-Boluda, J.C., Terol, M.J., et al. (2022) Multi-Body-Site Colonization Screening Cultures for Predicting Multi-Drug Resistant Gram-Negative and Gram-Positive Bacteremia in Hematological Patients. BMC Infectious Diseases, 22, Article No. 172. https://doi.org/10.1186/s12879-022-07154-3
[17]
Cusumano, J.A., Dupper, A.C., Malik, Y., Gavioli, E.M., Banga, J., Berbel Caban, A., et al. (2020) Staphylococcus aureus Bacteremia in Patients Infected with COVID-19: A Case Series. Open Forum Infectious Diseases, 7, ofaa518. https://doi.org/10.1093/ofid/ofaa518
[18]
Tayşi, M.R., Şentürk, G.Ç., Çalişkan, E., Öcal, D., Miroglu, G. and Şencan, İ. (2022) Implementation of the EUCAST Rapid Antimicrobial Susceptibility Test (RAST) Directly from Positive Blood Culture Bottles without the Advanced Identification Systems. Journal of Antimicrobial Chemotherapy, 77, 1020-1026. https://doi.org/10.1093/jac/dkac003
[19]
Jasuja, J.K., Zimmermann, S. and Burckhardt, I. (2020) Evaluation of EUCAST Rapid Antimicrobial Susceptibility Testing (RAST) for Positive Blood Cultures in Clinical Practice Using a Total Lab Automation. European Journal of Clinical Microbiology & Infectious Diseases, 39, 1305-1313. https://doi.org/10.1007/s10096-020-03846-3
[20]
Manyahi, J., Kibwana, U., Mgimba, E. and Majigo, M. (2020) Multi-Drug Resistant Bacteria Predict Mortality in Bloodstream Infection in a Tertiary Setting in Tanzania. PLOS ONE, 15, e0220424. https://doi.org/10.1371/journal.pone.0220424
[21]
Birru, M., Woldemariam, M., Manilal, A., Aklilu, A., Tsalla, T., Mitiku, A., et al. (2021) Bacterial Profile, Antimicrobial Susceptibility Patterns, and Associated Factors among Bloodstream Infection Suspected Patients Attending Arba Minch General Hospital, Ethiopia. Scientific Reports, 11, Article No. 15882. https://doi.org/10.1038/s41598-021-95314-x
[22]
Habyarimana, T., Murenzi, D., Musoni, E., Yadufashije, C. and Niyonzima, F.N. (2021) Bacteriological Profile and Antimicrobial Susceptibility Patterns of Bloodstream Infection at Kigali University Teaching Hospital. Infection and Drug Resistance, 14, 699-707. https://doi.org/10.2147/idr.s299520
[23]
Laupland, K.B. and Church, D.L. (2014) Population-Based Epidemiology and Microbiology of Community-Onset Bloodstream Infections. Clinical Microbiology Reviews, 27, 647-664. https://doi.org/10.1128/cmr.00002-14
[24]
Nagalo, A., Kabore, O.D., Koulbou, M., Sanogo, B., Yehouenou, C.L., Traore, I., et al. (2023) Automated Blood Culture Systems for Isolation of Bacterial Pathogens of Bloodstream Infection: The Experience of Bobo-Dioulasso Teaching Hospital, Burkina Faso. African Journal of Clinical and Experimental Microbiology, 24, 382-388. https://doi.org/10.4314/ajcem.v24i4.9
[25]
Wu, H., Yuan, E., Li, W., Peng, M., Zhang, Q. and Xie, K. (2022) Microbiological and Clinical Characteristics of Bloodstream Infections in General Intensive Care Unit: A Retrospective Study. Frontiers in Medicine, 9, Article 876207. https://doi.org/10.3389/fmed.2022.876207
[26]
Sands, K., Carvalho, M.J., Spiller, O.B., Portal, E.A.R., Thomson, K., Watkins, W.J., et al. (2022) Characterisation of Staphylococci Species from Neonatal Blood Cultures in Low-and Middle-Income Countries. BMC Infectious Diseases, 22, Article No. 593. https://doi.org/10.1186/s12879-022-07541-w
[27]
Kwiecinski, J.M. and Horswill, A.R. (2020) Staphylococcus aureus Bloodstream Infections: Pathogenesis and Regulatory Mechanisms. Current Opinion in Microbiology, 53, 51-60. https://doi.org/10.1016/j.mib.2020.02.005
[28]
Matsumoto, M., Kozakai, M., Furuta, R.A., Matsubayashi, K. and Satake, M. (2022) Association of Staphylococcus aureus in Platelet Concentrates with Skin Diseases in Blood Donors: Limitations of Cultural Bacterial Screening. Transfusion, 62, 621-632. https://doi.org/10.1111/trf.16804
[29]
Berry, K.A., Verhoef, M.T.A., Leonard, A.C. and Cox, G. (2022) Staphylococcus aureus Adhesion to the Host. Annals of the New York Academy of Sciences, 1515, 75-96. https://doi.org/10.1111/nyas.14807
[30]
Kahn, F., Resman, F., Bergmark, S., Filiptsev, P., Nilson, B., Gilje, P., et al. (2021) Time to Blood Culture Positivity in Staphylococcus aureus Bacteraemia to Determine Risk of Infective Endocarditis. Clinical Microbiology and Infection, 27, 1345.e7-1345.e12. https://doi.org/10.1016/j.cmi.2020.11.007
[31]
Poolman, J.T. and Anderson, A.S. (2018) Escherichia coli and Staphylococcus aureus: Leading Bacterial Pathogens of Healthcare Associated Infections and Bacteremia in Older-Age Populations. Expert Review of Vaccines, 17, 607-618. https://doi.org/10.1080/14760584.2018.1488590
[32]
Bonten, M., Johnson, J.R., van den Biggelaar, A.H.J., Georgalis, L., Geurtsen, J., de Palacios, P.I., et al. (2020) Epidemiology of Escherichia coli Bacteremia: A Systematic Literature Review. Clinical Infectious Diseases, 72, 1211-1219. https://doi.org/10.1093/cid/ciaa210
[33]
Dieng, A., Niang, M., Diallo, A.B., Faye, M., Lo, G., Ndiaye, S.M.L., et al. (2023) Etiology of Bacteremia under Haemodialysis Patients and Antibiotics Susceptibility Profile of Isolated Bacteria. Journal of Bacteriology and Virology, 53, 91-99. https://doi.org/10.4167/jbv.2023.53.2.091
[34]
Buonomini, A.R., Riva, E., Di Bonaventura, G. and Gherardi, G. (2020) Rapid Detection of Methicillin-Resistant Staphylococcus aureus Directly from Blood for the Diagnosis of Bloodstream Infections: A Mini-Review. Diagnostics, 10, Article 830. https://doi.org/10.3390/diagnostics10100830
[35]
Fernandez, J.M., Dobrick, J.B., Jadavji, A. and Adam, R.D. (2020) Staphylococcus aureus Bacteremia at a Referral Medical Center in Kenya: A Retrospective Review of Cases from 2010 to 2018. PLOS ONE, 15, e0234914. https://doi.org/10.1371/journal.pone.0234914
[36]
Bale, M.I., Babatunde, S. and Awe, S. (2021) Co-Infection of Methicillin Resistant Staphylococcus aureus Bacteremia and Malaria in Children Attending Cottage Hospital Ogidi Ilorin Kwara State, Nigeria. Saudi Journal of Pathology and Microbiology, 6, 306-310.
[37]
Ombelet, S., Kpossou, G., Kotchare, C., Agbobli, E., Sogbo, F., Massou, F., et al. (2022) Blood Culture Surveillance in a Secondary Care Hospital in Benin: Epidemiology of Bloodstream Infection Pathogens and Antimicrobial Resistance. BMC Infectious Diseases, 22, Article No. 119. https://doi.org/10.1186/s12879-022-07077-z
