A novel, fast-n-reliable, Quartz capillary silica zone electrophoresis required to upgrade an arsenal of analytical tools for current pharmacokinetics studies on porphyrin-fullerene nanoparticles has been proposed. The fullerene(C60)-tetra(p-hydroxyphenyl)porphyrin structures, known for their capabilities to paramagnetic 25Mg2+ delivery, were found quantitatively detectable in a total rat brain cytosol (S125) fraction, once these nanocationites administered in a single, either 1.0 mg/kg or 20.0 mg/kg, i.v. injection followed by 12 hrs long animal-drug exposition time. Driven by pressing needs in ongoing preclinical trial platform developments, the CZE track proposed might make an impact on both convenience and efficiency of pharmacokinetic estimate of medicinal nanocationites.
References
[1]
Rezayat, S.M., Boushehri, S.V.S., Salmanian, B., Omidvari, A.H., Tarighat, S., Esmaeili, S., et al. (2009) The Porphyrin-Fullerene Nanoparticles to Promote the ATP Overproduction in Myocardium: 25Mg2+-Magnetic Isotope Effect. EuropeanJournalofMedicinalChemistry, 44, 1554-1569. https://doi.org/10.1016/j.ejmech.2008.07.030
[2]
Mironov, A.F. (2011) Synthesis, Properties, and Potential Applications of Porphyrin-fullerenes. Macroheterocycles, 4, 186-208. https://doi.org/10.6060/mhc2011.3.08
[3]
Karmova, F.M., Lebedeva, V.S. and Mironov, A.F. (2016) Fullerene-Containing Porphyrins: Synthesis and Potential Practical Applications. RussianJournalofGeneralChemistry, 86, 2145-2179. https://doi.org/10.1134/s1070363216090322
[4]
Kazemzadeh, H. and Mozafari, M. (2019) Fullerene-Based delivery systems. DrugDiscoveryToday, 24, 898-905. https://doi.org/10.1016/j.drudis.2019.01.013
[5]
Amirshahi, N., Alyautdin, R.N., Sarkar, S., Rezayat, S.M., Orlova, M.A., Trushkov, I.V., et al. (2008) Fullerene-Based Low Toxic Nanocationite Particles (Porphyrin Adducts of Cyclohexyl Fullerene-C60) to Treat Hypoxia-Induced Mitochondrial Dysfunction in Mammalian Heart Muscle. ArchivesofMedicalResearch, 39, 549-559. https://doi.org/10.1016/j.arcmed.2008.05.007
[6]
(2013) Patent CN103193786A: Water-Soluble Fullerene—Porphyrin, Preparation Method and Application Thereof.
[7]
Buchachenko, A.L. (2015) Magneto-Biology and Medicine. Nova Biomedical Publishers Inc.
[8]
Buchachenko, A.L., Bukhvostov, A.A., Ermakov, K.V. and Kuznetsov, D.A. (2020) A Specific Role of Magnetic Isotopes in Biological and Ecological Systems. Physics and Biophysics Beyond. ProgressinBiophysicsandMolecularBiology, 155, 1-19. https://doi.org/10.1016/j.pbiomolbio.2020.02.007
[9]
Buchachenko, A.L. and Kuznetsov, D.A. (2014) Magnetic Control of Enzymatic Phosphorylation. JournalofPhysicalChemistry&Biophysics, 2, Article ID: 1000142. https://doi.org/10.4172/2161-0398.1000142
[10]
Koltover, V.K. (2014) Stable Magnetic Isotopes: From Spin Chemistry to Biomedicine. RussianChemicalBulletin, 63, 1029-1035. https://doi.org/10.1007/s11172-014-0545-3
[11]
Knape, M.J., Ballez, M., Burghardt, N.C., Zimmermann, B., Bertinetti, D., Kornev, A.P., et al. (2017) Divalent Metal Ions Control Activity and Inhibition of Protein Kinases. Metallomics, 9, 1576-1584. https://doi.org/10.1039/c7mt00204a
[12]
Buchachenko, A., Bukhvostov, A., Ermakov, K. and Kuznetsov, D. (2019) Nuclear Spin Selectivity in Enzymatic Catalysis: A Caution for Applied Biophysics. ArchivesofBiochemistryandBiophysics, 667, 30-35. https://doi.org/10.1016/j.abb.2019.04.005
[13]
Hudson, J.M., Golin, M.M. and Whiting, C. (1998) Capillary Zone Electrophoresis in a Comprehensive Screen for Drugs of Forensic Interest in Whole Blood: An Update. Journal of the Canadian Society of Forensic Science, 31, 1-29.
[14]
Hudson, J.C. (2014) CESI-MS Analysis of Bioliquids of Basic Drugs and Metabolites. Beckman Coulter Publisher.
[15]
Hudson, J.C. (2020) HPLC/LC-MS of Bioliquids. SCIEX Separations Booklette, Series 1B, 1584A-1598A. Beckman Coulter Publisher.
