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子宫内膜腺癌中piR-34377的表达及其临床病理意义
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Abstract:
目的：探讨piR-34377在子宫内膜腺癌及癌旁正常组织中的表达、临床病理意义和预后价值。方法：通过piRNA表达谱芯片筛选出子宫内膜腺癌及癌旁正常组织差异表达的piR-34377；选择69例子宫内膜腺癌通过qRT-PCR实验进行验证；分析piR-34377表达与子宫内膜腺癌患者临床病理特征和预后之间的关联。结果：piRNA表达谱测序结果得到包含piR-34377在内的2个piRNA表达水平下降。qRT-PCR实验验证结果表明，piR-34377 (1.962 ± 0.1152)表达水平明显低于癌旁正常组织(4.337 ± 0.2228)，t = 8.976，P < 0.05。子宫内膜腺癌组织中，piR-34377表达与淋巴结转移和分期密切相关(均P < 0.05)。并且piR-34377阴性表达组5年生存率低于正常或高表达组。单因素和多因素分析得出淋巴结转移和piR-34377表达水平可能是影响子宫内膜腺癌预后的独立危险因素(P < 0.05)。结论：piR-34377在子宫内膜腺癌组织中表达水平下调，piR-34377表达与淋巴结转移和分期密切相关和不良预后密切相关。
Objective: To investigate the expression, clinicopathological significance and prognostic value of piR-34377 in endometrial adenocarcinoma and adjacent normal tissues. Methods: piR-34377 differentially expressed in endometrial adenocarcinoma and adjacent normal tissues was screened by piRNA expression profile chip. Sixty-nine cases of endometrial adenocarcinoma were selected and verified by qRT-PCR. The correlation between piR-34377 expression and clinicopathological features and prognosis of patients with endometrial adenocarcinoma was analyzed. Results: The results of piRNA expression profile sequencing showed that the expression level of 2 piRNAs, including piR-34377, was decreased. qRT-PCR results showed that the expression level of piR-34377 (1.962 ± 0.1152) was significantly lower than that of adjacent normal tissues (4.337 ± 0.2228), t = 8.976, P < 0.05. In endometrial adenocarcinoma, piR-34377 expression was closely related to lymph node metastasis and stage (P < 0.05). The 5-year survival rate of piR-34377 negative expression group was lower than that of normal or high expression group. Univariate and multivariate analysis showed that lymph node metastasis and piR-34377 expression may be independent risk factors for the prognosis of endometrial adenocarcinoma (P < 0.05). Conclusions: The expression of piR-34377 is down-regulated in endometrial adenocarcinoma tissues. piR-34377 expression is closely related to lymph node metastasis, stage and poor prognosis.
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